歯周治療へのＢｏｎｅ　ｍｏｒｐｈｏｇｅｌ１ｅｔｉｃ　ｐｒｏｔｅｉｎ（ＢＭＰ）の応用に関する研究　リコンビナントＢＭＰ配合コラーゲン膜による骨形成の観察

DOI 被引用文献20件

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タイトル別名
  • A Study of the Application of Bone Morphogenetic Protein (BMP) to Periodontal Regenerative Therapy. A Histological Observation of Bone Formation by Recombinant Human BMP Combined with Collagen Membrane.
  • A Histological Observation of Bone Formation by Recombinant Human BMP Combined with Collagen Membrane
  • リコンビナントBMP配合コラーゲン膜による骨形成の観察

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The purpose of this study was to evaluate the ffect of using recombinant human BMP-2 (rhBMP -2) combined with a fibrous collagen membrane FCM) for periodontal therapy in adult rats.<BR>We originally developed FCM as a biodegradable barrier membrane for guided tissue regeneration techniques. It was made from telopeptide-depleted bovine skin collagen. In this study, we have made improvements for better manipulation, and used a new collagen membrane (FCM 3) as the carrier of rhBMP-2. The rhBMP-2 (0.5, 1.0, 2.0. μg), provided by Yamanouchi Pharmaceutical Co. Ltd., was precipitated onto approximately (0.5 mm.-4.0 mm mean weight 150 μg) of FCM 3, and the complex was then lyophilized and stored at . -70...<BR>The complex was implanted in dorsal subcutaneous and palateal subperiosteal sites of 20 adult Wistar rats (male, 12 weeks old). Newly formed bone was examined histologically after 21 days and 42 days of implantation. Some groups were assigned to the time course study, and bone formation was examined by a hard tissue labeling technique.<BR>The results showed that no ectopic bone formation had occurred in dorsal sites by the 21 st postoperative day. On the other hand, in palatal sites, new bone was observed around the carriers in 1.0μg and 2.0 . μg rhBMP-2 combined-groups. In t. hEe 2.0 . μg group, newly formed bone, that was connected with original palatal bone, covered the carrier, and the collagen fiber and bone matrix fiber were directly linked. Furthermore, the new bone was thick and the remaining carrier was fused with palatal bone on the 42 nd postoperative day. There were minimal inflammatory cells around the complex. No cartilage was found in any specimens.<BR>Using a hard tissue labeling technique, it was found that new bone formation promoted by rhBMP -2 may occur around the carrier as early as the 7 th postoperative day. On the 21 st day, new bone was seen not only on the surface but also in the carrier body.<BR>The results indicate that rhBMP-2 combined with a new collagen membrane (FCM 3) has good potential for application to periodontal regenerative therapy.

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