歯周疾患用軟膏剤のヒト歯肉由来培養ケラチノサイトに対する細胞毒性の量的比較

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  • Quantitative Comparison of Cytotoxicity of Dental Ointments for Periodontal Diseases to Normal Human Gingival Keratinocytes in Vitro.

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The cytotoxicities of dental ointments utilized for periodontal diseases were examined with normal human keratinocytes from gingival tissues by the uptake of neutral red (NR assay). The NR assay is a quantitative in vitro assay that distinguishes between viable, damaged or dead cells. Cultures from different individuals were established, and secondary cultures in serum-free medium were used. The cytotoxicity of ointments used on mucous membranes in the oral cavity was determined from the dose-response curves of inhibition of NR uptake in cells treated with ointments for 2 days. As a quantitative measure of cytotoxicity, NR 50, i. e., the concentration of ointments that resulted in a 50% decrease in NR uptake relative to untreated controls, was extrapolated from dose -response curves . The rank-order of cytotoxicities (NR 50) of injection type of ointments applied to periodontal pockets was HinoporonTM. a Tetra cortisone ointmentTM>PerioclineTM. ≈Terra -cortrill ointmentTM . The cytotoxicity of Hinoporon was approximately 1, 300-fold that of Terra -cortrill ointment . The rank-order of cytotoxicities of spread type of ointments was Dexaltin ointmentTM>Despa KOWATM>Tetracycline presteronTM. ≈Presteron. ≈KenalogTM. ≈AphtasoloneTM. If the same amount of ointments was applied to the oral mucosa for the same time, the lower-ranking ointments would be less cytotoxicity. These results not only provide useful estimates of relative toxicities of dental ointments to human cells, but also can be useful as a standard for screening of toxicities of the new products.

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