犬における血漿中プロベネシドの測定と血漿蛋白結合と薬物動態

DOI 日本農学文献記事索引 Web Site Web Site 参考文献22件
  • KAKIZAKI Takehiko
    Laboratory of Veterinary Radiology and Radiation Biology Kitasato University, School of Veterinary Medicine and Animal Sciences
  • YOKOYAMA Yukiko
    Laboratory of Veterinary Radiology and Radiation Biology Kitasato University, School of Veterinary Medicine and Animal Sciences
  • NATSUHORI Masahiro
    Laboratory of Veterinary Radiology and Radiation Biology Kitasato University, School of Veterinary Medicine and Animal Sciences
  • KARASAWA Azusa
    Laboratory of Veterinary Radiology and Radiation Biology Kitasato University, School of Veterinary Medicine and Animal Sciences
  • KUBO Satoshi
    Laboratory of Veterinary Radiology and Radiation Biology Kitasato University, School of Veterinary Medicine and Animal Sciences
  • YAMADA Naoaki
    Laboratory of Veterinary Radiology and Radiation Biology Kitasato University, School of Veterinary Medicine and Animal Sciences
  • ITO Nobuhiko
    Laboratory of Veterinary Radiology and Radiation Biology Kitasato University, School of Veterinary Medicine and Animal Sciences

書誌事項

タイトル別名
  • Probenecid: Its Chromatographic Determination, Plasma Protein Binding, and in vivo Pharmacokinetics in Dogs

この論文をさがす

抄録

Pharmacokinetics (PK) of probenecid including plasma probenecid concentrations, in vitro plasma protein binding properties, and in vivo PK parameters were determined in dogs. Probenecid concentrations were best determined by HPLC, which showed good linearity and good recovery with simple plasma preparation. The quantification limit of probenecid was approximately 50 ng/ml at S/N ratio = 3, by simple procedure with HCl and methanol treatment. Probenecid showed two types of binding characteristics, i.e., high-affinity with low-capacity and low-affinity with high-capacity binding. This result indicated 80-88% of probenecid was bound to plasma protein(s) at observed concentrations (< 80 μg/ml) in vivo at an intravenous dose of 20 mg/kg. Plasma probenecid concentration-time profile following i.v. administration in dogs showed biphasic decline and well fitted a two-compartment open model. The total body clearance was 0.34 ± 0.04 ml/min/kg, volume of distribution at steady-state was 0.46 ± 0.07 l/kg, elimination half-life was 18 ± 6 hr, and mean residence time (MRT) was 23 ± 6 hr. Since probenecid has been known as a potent inhibitor of renal tubular excretion of acidic drugs and highly binds to plasma proteins, our observation in relation to plasma protein binding and PK parameters will serve as the basic information concerning drug-drug interactions in dogs and in other mammalian species.<br>

収録刊行物

参考文献 (22)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ