Identification and Characterization of Wild Soybean (Glycine soja Sieb. et Zecc.) Strains with High Lutein Content

• Kanamaru Kyohei

  Laboratory of Plant Genetics and Evolution, Graduate School of Agriculture, Hokkaido University

• Wang Shaodong

  Laboratory of Plant Genetics and Evolution, Graduate School of Agriculture, Hokkaido University

• Abe Jun

  Laboratory of Plant Genetics and Evolution, Graduate School of Agriculture, Hokkaido University

• Yamada Tetsuya

  Laboratory of Plant Genetics and Evolution, Graduate School of Agriculture, Hokkaido University

• Kitamura Keisuke

  Laboratory of Plant Genetics and Evolution, Graduate School of Agriculture, Hokkaido University

抄録

Lutein is a lipophilic antioxidant vitamin compound that may be beneficial to health in humans. For this reason, increasing the content of lutein in crops and vegetables has become a major breeding objective. The genetic variability of the lutein content in soybean is poorly documented. We analyzed the lutein content of 1,100 accessions of wild and cultivated soybeans by high performance liquid chromatography (HPLC), and identified several wild soybean strains with a high content of lutein. These wild soybean strains, characterized by a deep yellow cotyledon, also displayed peaks of β-carotene and chlorophyll pigments. On the other hand, all the analyzed cultivated soybean accessions with a yellow cotyledon exhibited a small lutein peak and substantially no β-carotene and chlorophyll peaks, whereas those with a green cotyledon displayed chlorophyll peaks. In the present study, it was shown that most of the lutein content in the seeds of the three wild soybean strains with a high content of lutein, 00033715, 00112742 and B01167, originated from the cotyledon, while almost all the β-carotene and chlorophyll pigments were localized in the seed coat, but not in the cotyledon. These results were consistent with the presence of a deep yellow cotyledon, implying that it is possible to increase the lutein content in the cotyledon of soybean by using the high lutein content character of wild soybeans. Moreover, since the high lutein content was stably maintained in the seeds obtained from the wild soybean strains grown in 2004 and 2005, it was suggested that the high lutein content of the wild soybeans was a stable trait.<br>

収録刊行物

• Ｂｒｅｅｄｉｎｇ　Ｓｃｉｅｎｃｅ

  Ｂｒｅｅｄｉｎｇ　Ｓｃｉｅｎｃｅ 56 (3), 231-234, 2006

  日本育種学会

キーワード

• soybean
• wild soybean
• lutein
• genetic variability

詳細情報

• CRID: 1390282679698482048
• NII論文ID: 130004146060
• NII論文ID: 110004789102
• NII書誌ID: AA11317194
• DOI: 10.1270/jsbbs.56.231
• ISSN: 13473735
• ISSN: 13447610
• NDL書誌ID: 8037084
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8037084
• Web Site: http://www.jstage.jst.go.jp/article/jsbbs/56/3/56_3_231/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可