Estrogenic Activity of Phthalate Esters by In Vitro VTG Assay Using Primary-cultured Xenopus Hepatocytes

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Estrogenic activity of phthalate esters in dental soft resins was evaluated with an amphibian system consisting of a vitellogenin (VTG)-detecting Enzyme-Linked Immunosorbent Assay and a primary-cultured hepatocyte assay using adult male <I>Xenopus laevis</I>. In particular, phthalate esters — Di-n-butyl phthalate (DBP), Butyl phthalyl butyl glycolate (BPBG), Benzyl butyl phthalate (BBP), and Benzyl benzoate (BB) — were investigated. Bisphenol A (BPA) was prepared for comparison with these chemicals, and 17β-estradiol (E2) was used as a positive control. The chemicals were diluted in dimethyl sulfoxide (DMSO) to obtain final concentrations ranging from 10<SUP>-11</SUP> to 10<SUP>-4</SUP> mol/ l. BPA induced estrogenic activity at a concentration of 1.1×10<SUP>-6</SUP> mol/ l, while E2 showed at 4.1×10<SUP>-11</SUP> mol/ l. DBP, BBP, BB, and BPBG showed no estrogenic activity at concentrations between 4×10<SUP>-7</SUP> mol/ l and 1×10<SUP>-4</SUP> mol/ l. The latter result indicated that these phthalate esters might be metabolically transformed into non-estrogenic substances in <I>Xenopus</I> hepatocytes. Furthermore, this study demonstrated that through <I>in vitro</I> metabolism assessment, the estrogenic activity of chemical substances could be directly detected in terms of VTG secretion in primary-cultured <I>Xenopus</I> hepatocytes.


  • Dental Materials Journal

    Dental Materials Journal 25(3), 533-537, 2006-09-01

    The Japanese Society for Dental Materials and Devices

References:  19

Cited by:  2


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