Tension-dependent formation of stress fibers in fibroblasts: a study using semi-intact cells. JSME

• HIRATA Hiroaki

  Department of Molecular Physiology, National Institute for Physiological Science

• TATSUMI Hitoshi

  Department of Physiology, Nagoya University Graduate School of Medicine CREST, Japan Science and Technology Agency

• SOKABE Masahiro

  Department of Molecular Physiology, National Institute for Physiological Science Department of Physiology, Nagoya University Graduate School of Medicine ICORP, Cell Mechanosensing Project, Japan Science and Technology Agency

書誌事項

タイトル別名

• Tension-Dependent Formation of Stress Fibers in Fibroblasts: A Study Using Semi-Intact Cells

抄録

Stress fiber is a bundle of actin filaments, which also contains myosin and cross-linking proteins. It remains unclear how stress fibers are formed from their main components, actin and myosin. To address this question we examined the process of formation of stress fibers in a model system, a semi-intact cell. Fibroblasts were treated with a Rho kinase inhibitor to disorganize stress fibers. These cells were permeabilized and used as semi-intact cells in this study. When these cells were treated with ATP and Ca²⁺, stress fibers were restored within 15min. Motion analysis of actin filaments labeled with quantum dots during formation of stress fibers revealed actin filaments were gradually assembled while they were moving toward the center of the cell. This suggests that ATP-driven tension influences the stress fiber formation. The ATP-driven tension was mimicked in this study by artificial centripetal traction force, which was carried out by dragging the micropipette attached on the cell surface. Stress fibers were formed by the traction force application. These results suggest that tension in actomyosin meshwork is an important element in stress fiber formation.

収録刊行物

• JSME International Journal Series C Mechanical Systems, Machine Elements and Manufacturing

  JSME International Journal Series C Mechanical Systems, Machine Elements and Manufacturing 47 (4), 962-969, 2004

  一般社団法人 日本機械学会

キーワード

• Biomechanics
• Image Processing
• Optical Measurement
• Stress Fiber
• Semi-Intact Cell
• Traction Force
• Molecular Machine
• Self-Organization

詳細情報

• CRID: 1390001204678586880
• NII論文ID: 110004820824
• NII書誌ID: AA11179487
• DOI: 10.1299/jsmec.47.962
• ISSN: 1347538X
• ISSN: 13447653
• HANDLE: 2237/9066
• NDL書誌ID: 7190252
• Web Site: https://nagoya.repo.nii.ac.jp/records/7383
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7190252
• Web Site: http://www.jstage.jst.go.jp/article/jsmec/47/4/47_4_962/_pdf
• 本文言語コード: en
• データソース種別:
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  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可