Effect of shear stress on permeability of vascular endothelial monolayer cocultured with smooth muscle cells Effect of Shear Stress on Permeability of Vascular Endothelial Monolayer Cocultured with Smooth Muscle Cells

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Abstract

Effect of fluid shear stress on permeability of endothelial monolayer was investigated using an endothelial cell (EC)-smooth muscle cell (SMC) cocultured model (CM). Permeability of ECs to bovine serum albumin was measured after exposure to shear stress of 1.5Pa for 48 hours. Morphology and VE-cadherin expression of ECs in CM was almost same as of ECs cultured alone (monocultured model, MM). Under static condition, EC permeability was 5.1±3.0 × 10<sup>-6</sup>cm/sec (mean±SD) in MM and 6.5±3.4 × 10<sup>-6</sup>cm/sec in CM. After exposure to shear stress, EC permeability in CM (2.2±1.9 × 10<sup>-6</sup>cm/sec, <i>p</i> < 0.05) significantly decreased compared with the static model. However, EC permeability in MM (3.9±3.2 × 10<sup>-6</sup>cm/sec) did not significantly change compared with static cultured condition. These results suggested that cellular interactions between ECs and SMCs have important influences on EC permeability.

Journal

  • JSME International Journal Series C

    JSME International Journal Series C 47(4), 992-999, 2004-12-01

    The Japan Society of Mechanical Engineers

References:  29

Codes

  • NII Article ID (NAID)
    110004820828
  • NII NACSIS-CAT ID (NCID)
    AA11179487
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13447653
  • NDL Article ID
    7190317
  • NDL Source Classification
    ZN11(科学技術--機械工学・工業)
  • NDL Call No.
    Z53-Y272
  • Data Source
    CJP  NDL  NII-ELS  IR  J-STAGE 
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