The Effect of a Shear Flow on the Uptake of LDL and Ac-LDL by Cultured Vascular Endothelial Cells
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- NIWA Koichi
- Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture
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- KARINO Takeshi
- Laboratory of Biofluid Dynamics, Research Institute for Electronic Science, Hokkaido University
書誌事項
- タイトル別名
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- Effect of a Shear Flow on the Uptake of LDL and Ac LDL by Cultured Vascular Endothelial Cells
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The effects of a shear flow on the uptake of fluorescence-labeled low-density lipoprotein (DiI-LDL), acetylated LDL (DiI-Ac-LDL), and lucifer yellow (LY; a tracer of fluid-phase endocytosis) by cultured bovine aortic ECs were studied using a rotating-disk shearing apparatus. It was found that 2hours’ exposure of ECs to a laminar shear flow that imposed ECs an area-mean shear stress of 10dynes/cm2 caused an increase in the uptake of DiI-LDL and LY. By contrast, the uptake of DiI-Ac-LDL was decreased by exposure of the ECs to a shear flow. Addition of dextran sulfate (DS), a competitive inhibitor of scavenger receptors, reversed the effect of a shear flow on the uptake of DiI-Ac-LDL, resulting in an increase by the imposition of a shear flow, while the uptake of DiI-LDL and LY remained unaffected. It was concluded that a shear flow promotes the endocytosis of DiI-LDL and LY by ECs, but suppresses the uptake of DiI-Ac-LDL by ECs by inhibiting scavenger receptor-mediated endocytosis.
収録刊行物
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- JSME International Journal Series C Mechanical Systems, Machine Elements and Manufacturing
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JSME International Journal Series C Mechanical Systems, Machine Elements and Manufacturing 48 (4), 436-443, 2005
一般社団法人 日本機械学会
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詳細情報 詳細情報について
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- CRID
- 1390001204680777600
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- NII論文ID
- 110004826662
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- NII書誌ID
- AA11179487
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- ISSN
- 1347538X
- 13447653
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- NDL書誌ID
- 7741707
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 使用不可