Distinct Modes of Cell Death by Ionizing Radiation Observed in Two Lines of Feline T-Lymphocytes

• KAKIZAKI Takehiko

  Laboratory of Veterinary Radiology and Radiation Biology, Department of Veterinary Medicine, Kitasato University Graduate School of Veterinary Medicine and Animal Sciences Microbeam Radiation Biology Group, Japan Atomic Energy Agency

• HAMADA Nobuyuki

  Microbeam Radiation Biology Group, Japan Atomic Energy Agency Department of Quantum Biology, Division of Bioregulatory Medicine, Gunma University Graduate School of Medicine

• WADA Seiichi

  Microbeam Radiation Biology Group, Japan Atomic Energy Agency

• FUNAYAMA Tomoo

  Microbeam Radiation Biology Group, Japan Atomic Energy Agency

• SAKASHITA Tetsuya

  Microbeam Radiation Biology Group, Japan Atomic Energy Agency

• HOHDATSU Tsutomu

  Laboratory of Veterinary Radiology and Radiation Biology, Department of Veterinary Medicine, Kitasato University Graduate School of Veterinary Medicine and Animal Sciences

• SANO Tadashi

  Laboratory of Veterinary Radiology and Radiation Biology, Department of Veterinary Medicine, Kitasato University Graduate School of Veterinary Medicine and Animal Sciences

• NATSUHORI Masahiro

  Laboratory of Veterinary Radiology and Radiation Biology, Department of Veterinary Medicine, Kitasato University Graduate School of Veterinary Medicine and Animal Sciences

• KOBAYASHI Yasuhiko

  Microbeam Radiation Biology Group, Japan Atomic Energy Agency Department of Quantum Biology, Division of Bioregulatory Medicine, Gunma University Graduate School of Medicine

• ITO Nobuhiko

  Laboratory of Veterinary Radiology and Radiation Biology, Department of Veterinary Medicine, Kitasato University Graduate School of Veterinary Medicine and Animal Sciences

Abstract

We have examined in vitro radiosensitivities and radioresponses to ⁶⁰Co γ-rays irradiation in feline T-lymphocyte cell lines, FeT-J and FL-4. There seemed to be no significant difference in clonogenic survival between the two lines. The mean lethal dose for both was both 1.9 Gy, and surviving fraction at 2 Gy was 0.30 and 0.48 for FeT-J and FL-4 cells, respectively. However, TUNEL assay indicated much higher degrees of apoptosis induction in FeT-J cells (>40%) than in FL-4 cells (<10%) at 4 days after 15 Gy irradiation. Microscopic examination revealed a larger population of multi-nucleate cells in FL-4 cells (60.3%) than in FeT-J cells (16.0%) at 4 days after 15 Gy irradiation, suggesting that a larger ratio of mitotic catastrophe occurred in FL-4 cells. These results suggest that FeT-J is more likely to be induced into apoptosis and FL-4 is more likely to fall into mitotic catastrophe, and eventually necrosis; both of them showed a similar surviving fraction against γ-rays. The results also indicate that FL-4 cells follow a process other than apoptosis to cell death, suggesting the presence of a regulatory mechanism that may control the relationship between mitotic catastrophe and apoptosis in feline T-lymphocytes.<br>

Journal

• Journal of Radiation Research

  Journal of Radiation Research 47 (3/4), 237-243, 2006

  Journal of Radiation Research Editorial Committee

Keywords

• Feline T-lymphocytes
• Apoptosis
• Mitotic catastrophe
• Clonogenic survival
• Ionizing radiation

Details

• CRID: 1390282680194541568
• NII Article ID: 110004850770
• NII Book ID: AA00705792
• DOI: 10.1269/jrr.0618
• ISSN: 13499157
• ISSN: 04493060
• Web Site: http://academic.oup.com/jrr/article-pdf/47/3-4/237/2824300/jrr-47-237.pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10918839
• Web Site: https://search.jamas.or.jp/link/ui/2007159722
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • NDL-Digital
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed