金基板上でのグアニン塩基の酸化電流に基づくDNA量の測定  [in Japanese] Measurement of DNA Amount on Gold Plate Based on the Oxidation Current of Guanine  [in Japanese]

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Author(s)

    • 矢吹 聡一 YABUKI Soichi
    • 産業技術総合研究所生物機能工学研究部門 National Institute of Advanced Industrial Science and Technology (AIST)
    • 佐藤 縁 SATO Yukari
    • 産業技術総合研究所生物機能工学研究部門 National Institute of Advanced Industrial Science and Technology (AIST)
    • 丹羽 修 NIWA Osamu
    • 産業技術総合研究所生物機能工学研究部門 National Institute of Advanced Industrial Science and Technology (AIST)

Abstract

Electrochemical oxidation on a nucleic acid base, such as guanine, adenine, thymine, cytosine, was examined; only guanine could be oxidized on a gold electrode. Because deoxyguanosine, deoxyguanosine monophosphate were also oxidized on the electrode, guanine in DNA would be oxidized on the electrode. To prove DNA oxidation, thiolated-single strand DNA (ssDNA) was immobilized on a gold electrode, and electrochemical measurements were performed: differential pulse voltammetry (DPV) and chronoamperometry were examined. The voltammogram of DPV on an ssDNA-modified electrode had an oxidation peak at +1.17 V <i>vs</i>. Ag/AgCl. The oxidation current was caused by the oxidation of guanine in the DNA-base. The electrical charge difference between a modified electrode and a bare electrode on chronoamperometry agreed with the guanine amount of ssDNA on gold, which was obtained from the result of a quartz crystal microbalance.<br>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 55(12), 975-978, 2006-12-05

    The Japan Society for Analytical Chemistry

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Codes

  • NII Article ID (NAID)
    110004997512
  • NII NACSIS-CAT ID (NCID)
    AN00222633
  • Text Lang
    JPN
  • Article Type
    NOT
  • ISSN
    05251931
  • NDL Article ID
    8605266
  • NDL Source Classification
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL Call No.
    Z17-9
  • Data Source
    CJP  NDL  NII-ELS  J-STAGE  NDL-Digital 
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