Determination of P-Glycoprotein ATPase Activity Using Luciferase
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- Matsunaga Tamihide
- Division of Pharmacy, Shinshu University Hospital
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- Kose Eiji
- Division of Pharmacy, Shinshu University Hospital
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- Yasuda Sachiyo
- Division of Pharmacy, Shinshu University Hospital
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- Ise Hirohiko
- Department of Organ Regeneration, Institute of Organ Transplants, Reconstructive Medicine and Tissue Engineering, Shinshu University Graduate School of Medicine
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- Ikeda Uichi
- Department of Organ Regeneration, Institute of Organ Transplants, Reconstructive Medicine and Tissue Engineering, Shinshu University Graduate School of Medicine
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- Ohmori Shigeru
- Division of Pharmacy, Shinshu University Hospital
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Abstract
We investigated whether P-glycoprotein (P-gp) ATPase activity of Caco-2 cell membranes could be estimated by measuring consumption of ATP using luciferin–luciferase reaction, and whether the results would be useful for assessment of the interactions between P-gp and drugs. The vanadate-sensitive ATPase activity of Caco-2 cell membranes was measured rapidly with high sensitivity using luciferin–luciferase reaction. Cyclosporin A, verapamil, digoxin and quinidine stimulated the ATPase activity concentration-dependently with Km values of 5.3, 0.9, 1.2 and 4.1 μM, respectively. These values except for digoxin were comparable with previous reports. The ATPase activity and P-gp mRNA expression in Caco-2 cells were induced by all-trans-retinoic acid, digoxin and levothyroxine, but not dexamethasone or rifampicin. This method was useful to assess interactions with P-gp and drugs, and was used to elucidate the mechanisms of interaction of levothyroxine and digoxin.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 29 (3), 560-564, 2006
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390282679601236224
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- NII Article ID
- 110005602145
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- NII Book ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 7830585
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed