Delivery of Condensed DNA by Liposomal Non-viral Gene Delivery System into Nucleus of Dendritic Cells

• Nakamura Takashi

  Graduate School of Pharmaceutical Sciences, Hokkaido University Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)

• Moriguchi Rumiko

  Graduate School of Pharmaceutical Sciences, Hokkaido University Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)

• Kogure Kentaro

  Graduate School of Pharmaceutical Sciences, Hokkaido University Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)

• Minoura Arisa

  Graduate School of Pharmaceutical Sciences, Hokkaido University

• Masuda Tomoya

  Graduate School of Pharmaceutical Sciences, Hokkaido University Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)

• Akita Hidetaka

  Graduate School of Pharmaceutical Sciences, Hokkaido University Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)

• Kato Kazunori

  Department of Molecular Medicine, Sapporo Medical University

• Hamada Hirofumi

  Department of Molecular Medicine, Sapporo Medical University

• Ueno Masaharu

  Faculty of Pharmaceutical Sciences, University of Toyama

• Futaki Shiroh

  Institute for Chemical Research, Kyoto University Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST)

• Harashima Hideyoshi

  Graduate School of Pharmaceutical Sciences, Hokkaido University Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST)

Abstract

In this study, we developed novel double-membranous non-viral gene delivery system modified with SV-40 T antigen-derived nuclear localization signal (NLS-DMEND) for delivery of luciferase plasmid DNA to nucleus of non-dividing mouse bone marrow-derived dendritic cells (BMDC). Intracellular trafficking and gene expression of NLS-DMEND in the BMDC were evaluated. Condensed DNA was observed in the nucleus by confocal laser scanning microscopy, and the NLS-DMEND induced significant luciferase activity in the BMDC. It was suggested that the condensed DNA particle transferred into nucleus via energy dependent manner, since the nuclear transfer was inhibited by metabolic inhibitors. In conclusion, condensed plasmid DNA was delivered into the nucleus of non-dividing BMDC by NLS-DMEND.

Journal

• Biological and Pharmaceutical Bulletin

  Biological and Pharmaceutical Bulletin 29 (6), 1290-1293, 2006

  The Pharmaceutical Society of Japan

Keywords

• non-viral gene delivery system
• non-dividing cell
• nuclear localization signal
• nuclear transfer

Details

• CRID: 1390282679604257664
• NII Article ID: 110005602287
• NII Book ID: AA10885497
• DOI: 10.1248/bpb.29.1290
• ISSN: 13475215
• ISSN: 09186158
• NDL BIB ID: 7921905
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7921905
• Web Site: http://www.jstage.jst.go.jp/article/bpb/29/6/29_6_1290/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2007004729
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed