Efficient Gene Transfer into Differentiated Human Trophoblast Cells with Adenovirus Vector Containing RGD Motif in the Fiber Protein
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- Koizumi Naoya
- Laboratory of Gene Transfer and Regulation, National Institute of Biomedical Innovation, Japan Department of Pharmaceutics and Biopharmaceutics, Showa Pharmaceutical University
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- Mizuguchi Hiroyuki
- Laboratory of Gene Transfer and Regulation, National Institute of Biomedical Innovation, Japan Graduate School of Pharmaceutical Sciences, Osaka University
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- Kondoh Masuo
- Graduate School of Pharmaceutical Sciences, Osaka University Department of Pharmaceutics and Biopharmaceutics, Showa Pharmaceutical University
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- Fujii Makiko
- Department of Pharmaceutics and Biopharmaceutics, Showa Pharmaceutical University
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- Nakanishi Tsuyoshi
- Department of Toxicology, Graduate School of Pharmaceutical Sciences, Osaka University
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- Utoguchi Naoki
- Department of Biopharmaceutics, School of Pharmaceutical Sciences, Teikyo University
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- Watanabe Yoshiteru
- Department of Pharmaceutics and Biopharmaceutics, Showa Pharmaceutical University
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Abstract
Previously we reported fiber-modified adenovirus (Ad) vectors containing the Arg-Gly-Asp (RGD) motif on the HI loop of the fiber knob (Ad-RGD vectors) have high gene transfer efficacy into some human trophoblast cell lines. In the current study, we investigate transgene activity of Ad-RGD during differentiation of human cytotrophoblast BeWo cells into syncytiotrophoblast-like cells. Although cellular differentiation into syncytiotrophoblast cells was followed by a decrease in the coxsackievirus and adenovirus receptor levels on the cell membrane, the αVβ3 and αVβ5 integrin levels did not change. Conventional adenovirus vector had lower transduction activity in the differentiated cells than non-differentiated cells. In contrast, Ad-RGD vector had no influence on differentiation and had a ca. 2—5 fold higher transduction activity than that of the conventional Ad vector. Thus, Ad-RGD vector can be a powerful tool for gene transfer experiments in syncytiotrophoblast cells.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 29 (6), 1297-1299, 2006
The Pharmaceutical Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390282679604255232
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- NII Article ID
- 110005602289
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- NII Book ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 7921924
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed