Killing of Feline T-Lymphocytes by Gamma-Rays and Energetic Carbon Ions

• KAKIZAKI Takehiko

  Department of Veterinary Medicine, Kitasato University Graduate School of Veterinary Medicine and Animal Sciences Microbeam Radiation Biology Group, Japan Atomic Energy Agency

• HAMADA Nobuyuki

  Microbeam Radiation Biology Group, Japan Atomic Energy Agency Department of Quantum Biology, Division of Bioregulatory Medicine, Gunma University Graduate School of Medicine

• FUNAYAMA Tomoo

  Microbeam Radiation Biology Group, Japan Atomic Energy Agency

• SAKASHITA Tetsuya

  Microbeam Radiation Biology Group, Japan Atomic Energy Agency

• WADA Seiichi

  Microbeam Radiation Biology Group, Japan Atomic Energy Agency

• HOHDATSU Tsutomu

  Department of Veterinary Medicine, Kitasato University Graduate School of Veterinary Medicine and Animal Sciences

• NATSUHORI Masahiro

  Department of Veterinary Medicine, Kitasato University Graduate School of Veterinary Medicine and Animal Sciences

• SANO Tadashi

  Department of Veterinary Medicine, Kitasato University Graduate School of Veterinary Medicine and Animal Sciences

• KOBAYASHI Yasuhiko

  Microbeam Radiation Biology Group, Japan Atomic Energy Agency Department of Quantum Biology, Division of Bioregulatory Medicine, Gunma University Graduate School of Medicine

• ITO Nobuhiko

  Department of Veterinary Medicine, Kitasato University Graduate School of Veterinary Medicine and Animal Sciences

Abstract

High linear energy transfer (LET) heavy charged particles have previously been applied clinically to human cancer radiotherapy because of their excellent physical properties of selective dose distribution and higher relative biological effectiveness (RBE) for human; however, such an approach has yet to be applied to cat patients. The present study investigates the biological effectiveness of low-LET γ-rays (0.2 keV/μm) compared to high-LET carbon ions (114 keV/μm) in feline T- lymphocyte FeT-J cells. Clonogenic survival analysis revealed that the RBE value of carbon ions was 2.98 relative to a 10% survival dose (D₁₀) by γ-rays, and that the inactivation cross-section in cells exposed to γ-rays and carbon ions was 0.023 and 38.9 μm², respectively. Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labeling (TUNEL) analysis revealed that TUNEL-positive frequency in carbon-irradiation cells is higher than for γ-irradiated cells against exposure to the same physical doses, but that very little difference in TUNEL-positive frequency is observed between cells exposed to the respective D₁₀ dose of γ-rays. Our data thus indicate that carbon ions are more effective for cell killing than γ-rays at the same physical doses, but kill cells to an extent that is comparable to γ-rays at the same biological doses. Carbon ion radiotherapy is therefore a promising modality for cat patients.<br>

Journal

• Journal of Veterinary Medical Science

  Journal of Veterinary Medical Science 68 (12), 1269-1273, 2006

  JAPANESE SOCIETY OF VETERINARY SCIENCE

Keywords

• accelerated carbon ions
• apoptosis
• feline T lymphocytes
• gamma-ray
• relative biological effect

Details

• CRID: 1390001206428804352
• NII Article ID: 110005716367
• NII Book ID: AA10796138
• DOI: 10.1292/jvms.68.1269
• ISSN: 13477439
• ISSN: 09167250
• NDL BIB ID: 8626740
• Web Site: http://id.ndl.go.jp/bib/8626740
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8626740
• Web Site: http://www.jstage.jst.go.jp/article/jvms/68/12/68_12_1269/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2007174881
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed