Expression of WISP-1 (ccn4), WISP-2 (ccn5) and WISP-3 (ccn6) in Rheumatoid Arthritic Synovium Evaluated by DNA Microarrays

• Mori Hiroki

  Department of Clinical Diagnosis, Shujitsu University School of Pharmacy

• Nishida Keiichiro

  Department of Orthopaedics, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

• Ozaki Toshifumi

  Department of Orthopaedics, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

• Inoue Hajime

  Department of Orthopaedics, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

• Setsu Kojun

  Laboratories for Structure and Function of Pharmaceutical Sciences, Tokushima Bunri University

• Tsujigiwa Hidetsugu

  Deparment of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

• Nagatsuka Hitoshi

  Deparment of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

• Gunduz Mehmet

  Deparment of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University

• Nakanishi Tohru

  Department of Clinical Diagnosis, Shujitsu University School of Pharmacy

抄録

Rheumatoid disease (RA) is one of the complex diseases that showed multiple progression stages. At first inflammatory response is occurred by self antibodies, and then the proliferation of synoviocytes is abnormally promoted. These synoviocytes invade into articular structures and finally articular cartilage is destroyed. In order to analyze growth-promoting factors in synovial cells from articular tissues of RA, we performed DNA microarray analysis and compared the expression patterns of their mRNA to that of synoviocytes from osteoarthritis (OA). The results showed that the expression of many genes was up-regulated in RA synoviocytes comparing with OA synoviocytes. Among these genes, RT-PCR analysis revealed that Wnt-1-induced secreted protein 2 (WISP-2, CCN5) and protein 3 (WISP-3, CCN6) which are belong to the novel gene family, CCN, were highly up-regulated in RA synoviocytes. In addition, one truncated isoform of WISP-3 which may be related to the ongoing of some type of RA, was detected in RA synoviocytes. Furthermore, immunohistochemical analysis showed that the products of both CCN genes were highly distributed in active synoviocytes of articular tissues of RA. These results indicate that both CCN genes and their products play some roles in the progression of RA.

収録刊行物

• Journal of Hard Tissue Biology

  Journal of Hard Tissue Biology 15 (3), 89-95, 2006

  硬組織再生生物学会

キーワード

• Rheumatoid arthritis
• Synovial cells
• Microarray
• WISP gene
• CCN family

詳細情報

• CRID: 1390001204457166976
• NII論文ID: 110006151628
• NII書誌ID: AA11074332
• DOI: 10.2485/jhtb.15.89
• COI: 1:CAS:528:DC%2BD2sXhvVCru7s%3D
• ISSN: 1880828X
• ISSN: 13417649
• Web Site: https://search.jamas.or.jp/link/ui/2007191489
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可