A Calcium-dependent Protein Kinase (CDPK) in the Unicellular Green Alga Closterium ehrenbergii

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  • Yuasa Takashi
    Department of Plant Resources, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
  • Hashimoto Haruki
    Department of Life and Cognitive Sciences, Graduate School of Arts and Sciences, The University of Tokyo

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  • Calcium dependent Protein Kinase CDPK in the Unicellular Green Alga Closterium ehrenbergii

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Abstract

An in-gel kinase assay revealed that homogenates of Closterium ehrenbergii contain Ca2+-dependent protein kinases (CDPKs) ranging in relative molecular mass from 47 to 60 kDa, that phosphorylated histone H1, myelin basic protein, and casein as substrates in a Ca2+-dependent manner. Immunoblotting and immunoprecipitation assays of Closterium extracts using anti-Dunaliella CDPK serum showed that the antibody cross-reacted with a Closterium CDPK with a relative molecular mass of 55 kDa in the soluble fraction. These results indicate that Closterium possesses CDPK-related polypeptides: one is a 55-kDa CDPK that is immunologically related to Dunaliella CDPK and the others have various molecular masses.<br>

Journal

  • Microbes and Environments

    Microbes and Environments 21 (4), 278-283, 2006

    Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

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