Genomic Structure and Expression of the Soluble Guanylyl Cyclase α_2 Subunit Gene in the Medaka Fish Oryzias latipes

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Author(s)

    • Yao Yuko YAO Yuko
    • Division of Biological Sciences, Graduate School of Science, Hokkaido University Division of Biological Sciences, Graduate School of Science, Hokkaido University
    • Yamamoto Takehiro YAMAMOTO Takehiro
    • Division of Biological Sciences, Graduate School of Science, Hokkaido University Division of Biological Sciences, Graduate School of Science, Hokkaido University
    • Tsutsumi Makiko [他] TSUTSUMI Makiko
    • Division of Biological Science, Graduate School of Science, Nagoya University Division of Biological Science, Graduate School of Science, Nagoya University
    • MATSUDA Masaru
    • Laboratory of Reproductive Biology, National Institute for Basic Biology Laboratory of Reproductive Biology, National Institute for Basic Biology
    • HORI Hiroshi
    • Division of Biological Science, Graduate School of Science, Nagoya University Division of Biological Science, Graduate School of Science, Nagoya University
    • NARUSE Kiyoshi
    • Department of Biological Sciences, Graduate School of Science, University of Tokyo Department of Biological Sciences, Graduate School of Science, University of Tokyo
    • MITANI Hiroshi
    • Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo Department of Integrated Biosciences, Graduate School of Frontier Sciences University of Tokyo
    • SHIMA Akihiro
    • Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo Department of Integrated Biosciences, Graduate School of Frontier Sciences University of Tokyo
    • ASAKAWA Shuichi
    • Department of Molecular Biology, Keio University School of Medicine Department of Molecular Biology, Keio University School of Medicine
    • SHIMIZU Nobuyoshi
    • Department of Molecular Biology, Keio University School of Medicine Department of Molecular Biology, Keio University School of Medicine
    • SUZUKI Norio
    • Division of Biological Sciences, Graduate School of Science, Hokkaido University Division of Biological Sciences, Graduate School of Science, Hokkaido University

Abstract

A cDNA clone encoding the soluble guanylyl cyclase α2 subunit was isolated from medaka fish (Oryzias latipes) and designated as OlGCS-α2. The OlGCS-α2 cDNA was 3192 bp in length and the open reading frame (ORF) encodes a protein of 805 amino acids. The deduced amino acid sequence has high similarity to that of the mammalian α2 subunit gene except for the N-terminal regulatory domain. The C-terminal 5 amino acids, "RETSL", which have been reported to interact with the post synaptic density protein (PSD)-95 were conserved. An RNase protection assay with adult fish organs showed that OlGCS-α2 was expressed mainly in the brain and testis. The complete nucleotide sequence (about 41 kbp) of the OlGCS-α2 genomic DNA clone isolated from a medaka fish BAC library indicated that the OlGCS-α2 gene consisted of 9 exons and 8 introns. The 5-flanking region and larger introns, such as introns 1, 4, and 7, contained the several fragments conserved in the nucleotide sequences of Rex6 (non-long terminal repeat retrotransposon), MHC class I genomic region, and OlGC1, the medaka fish homolog of the mammalian guanylyl cyclase B gene. Linkage analysis on the medaka fish chromosome demonstrated that the OlGCS-α2 gene was mapped to LG13; this mapping position was different from those for the OlGCS-α1 and OlGCS-β1 genes (LG1).

Journal

  • ZOOLOGICAL SCIENCE

    ZOOLOGICAL SCIENCE 20(10), 1293-1304, 2003-10-25

    The Zoological Society of Japan

References:  38

Cited by:  1

Codes

  • NII Article ID (NAID)
    110006162813
  • NII NACSIS-CAT ID (NCID)
    AA10545874
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    02890003
  • NDL Article ID
    6718545
  • NDL Source Classification
    ZR4(科学技術--生物学--動物)
  • NDL Call No.
    Z53-Y164
  • Data Source
    CJP  CJPref  NDL  NII-ELS  IR  J-STAGE  NDL-Digital 
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