Distinct Glycosylation in Interstitial and Serum Tenascin-X

DOI HANDLE Web Site Web Site 被引用文献1件 参考文献47件 オープンアクセス
  • Kinoshita Takeshi
    Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University
  • Ariga Hiroyoshi
    Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University
  • Matsumoto Ken-ichi
    Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University

この論文をさがす

抄録

We developed an easy and fast method to isolate extracellular matrix tenascin-X (TNX) from various tissues in mice based on TNX antibody affinity purification. We purified approximately 350-kDa cellular interstitial TNX (iTNX) from the spleen, liver and kidney as well as 200-kDa serum TNX (sTNX). Since the nature and significance of glycosylation in TNX remains to be elucidated, glycobiochemical properties of purified TNX were characterized by lectin blot analysis. Lectin blots by Con A, LCA, PHA-E4, RCA120 or WGA revealed the presence of N-glycan in the cellular TNX and especially complex-type N-glycan in the serum TNX. In addition, the iTNX from liver and kidney also possessed O-glycan based on the reaction to PNA. The binding to AAL indicated that iTNX from the three tissues possesses fucose linked α1,6 to a pentasaccharide core, whereas sTNX does not. The reaction to SSA but not to MAM suggested the presence of sialic acid linked α2,6 to galactose in both cellular and serum TNX. Lectin blots of trypsin-treated iTNX from the spleen also demonstrated that WGA alone reacts to the t300 product derived from the amino-terminal 300-kDa portion.

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (47)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ