High inoculation cell density could accelerate the differentiation of human bone marrow mesenchymal stem cells to chondrocyte cells.

• Takagi, Mutsumi

• Umetsu, Yousuke

• Fujiwara, Masashi

• Wakitani, Shigeyuki

Abstract

The effects of the density of human mesenchymal stem cells (MSCs) on their differentioation to chondrocytes in a differentiation medium supplemented with dexamethasone, TGFβ3, and IGF-1 were investigated for the regenerative therapy of cartilage. The increase in the initial density of MSCs from 0.05×104 to 0.9×104 cells/cm2 accelerated the increase in the expression level of aggrecan mRNA during the differentiation culture for 7 d. The conditioned medium harvested at 7 d from the differentiation culture with an initial MSC density of 0.3×104 cells/cm2 accelerated the initial increase in the expression level for 3 d in the differentiation culture with an initial MSC density of 0.3×104 cells/cm2, whereas the conditioned medium harvested at 7 d in the differentiation culture with an initial MSC density of 0.05×104 cells/cm2 did not. The differentiation culture after 14 d with an initial MSC concentration of 0.3×104 cells/cm2 showed an expression level 1.7-fold that in the case of the culture with an initial MSC concentration of 0.05×104 cells/cm2. Thus, a high MSC inoculum density might be appropriate for the rapid differentiation of MSCs to chondrocytes.

Journal

• Journal of Bioscience and Bioengineering

  Journal of Bioscience and Bioengineering 103 (1), 98-100, 2007-01

  The Society for Biotechnology, Japan

Keywords

• cell density
• mesenchymal stem cell
• differentiation
• chondrocyte

Details

• CRID: 1050845763915970432
• NII Article ID: 110006164023
• NII Book ID: AA11307678
• ISSN: 13474421
• ISSN: 13891723
• HANDLE: 2115/22086
• NDL BIB ID: 8616229
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8616229
• Web Site: https://search.jamas.or.jp/link/ui/2007161765
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • NDL
  • CiNii Articles