Effect of collagen immobilized poly(ethylene-co-vinyl alcohol) to human pulp derived cell

DOI
  • NISHITANI Yoshihiro
    Department of Operative Dentistry Okayama University Graduate School of Medicine and Dentistry
  • TAKAGI Ryo
    Department of Operative Dentistry Okayama University Graduate School of Medicine and Dentistry
  • USHIRO Naoki
    Department of Operative Dentistry Okayama University Graduate School of Medicine and Dentistry
  • ITOTA Toshiyuki
    Department of Operative Dentistry Okayama University Graduate School of Medicine and Dentistry
  • HYON Suong-Hyu
    Instutute for Frontier Medical Science, Kyoto University
  • TSUTSUMI Sadami
    Instutute for Frontier Medical Science, Kyoto University
  • YOSHIYAMA Masahiro
    Department of Operative Dentistry Okayama University Graduate School of Medicine and Dentistry

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Other Title
  • コラーゲン固定化エチレン—ビニルアルコール共重合体(EVA)のヒト歯髄由来細胞に対する影響

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Abstract

The aim of this study was to investigate the morphology, growth and type I collagen expression of human pulp derived cells on the poly(ethylene-co-vinyl alcohol) (EVA) coated with collagen (+C) film. Cell morphology and growth curve for EVA+C group was similar to that for control group, which was cultured on the normal cell culture dish. However, for EVA group cell shape showed no extension and the growth was down-regulated. There was no difference in expression of type I collagen in the cultured cells among the three groups.<br>These results suggested that the immobilization of collagen to EVA facilitate an attachment and extension of human pulp derived cells on the EVA film without down regulation of type I collagen.

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