Immobilization of Fibronectin onto Organic Hexamethyldisiloxane Coatings with Plasma Surface Modification

• YOSHINARI Masao

  Department of Dental Materials Science and Oral Health Science Center, Tokyo Dental College

• HAYAKAWA Tohru

  Department of Dental Materials, Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo

• MATSUZAKA Kenichi

  Department of Clinical Pathophysiology, Oral Health Science Center, Tokyo Dental College

• INOUE Takashi

  Department of Clinical Pathophysiology, Oral Health Science Center, Tokyo Dental College

• ODA Yutaka

  Department of Dental Materials Science and Oral Health Science Center, Tokyo Dental College

• SHIMONO Masaki

  Department of Pathology, Oral Health Science Center, Tokyo Dental College

Bibliographic Information

Other Title

• <b>—Analysis of Fibronectin Adsorption Using Quartz Crystal Microbalance-dissipation Technique—</b>

Abstract

The immobilization behavior of fibronectin onto plasma-surface modified titanium was evaluated by using the quartz crystal microbalance-dissipation (QCM-D) technique. The plasma-surface modifications included a thin-film coating of hexamethyldisiloxane (HMDSO) and an O₂-plasma treatment on titanium. The contact angle of HMDSO-coatings against double-distilled water was 100 degrees, and dramatically decreased after the O₂-plasma treatment with less than 5 degrees. XPS analysis indicated the introduction of an O₂-functional group onto the HMDSO surface by the O₂-plasma treatment. The QCM-D technique enabled evaluation the adsorption behavior of fibronectin. The largest amount of fibronectin adsorbed was on both as-coated HMDSO (hydrophobic surfaces) and O₂-plasma treated at 10-min (hydrophilic surfaces) surfaces compared to O₂-plasma treated (24-h) surfaces. These results indicated that two adsorption mechanisms of the fibronectin to substrates were considered, including hydrophobic interaction and ionic bond via OH group and/or O₂-functional group concomitant with hydrophilic surfaces.

Journal

• Journal of Oral Tissue Engineering

  Journal of Oral Tissue Engineering 1 (1), 69-79, 2004

  Japanese Association of Regenerative Dentistry

Keywords

• Surface modification
• Plasma polymerization
• Adsorption
• Fibronectin
• Quartz crystal microbalance

Details

• CRID: 1390282680203562624
• NII Article ID: 110006164147
• NII Book ID: AA1195240X
• DOI: 10.11223/jarde.1.69
• ISSN: 18800823
• ISSN: 13489623
• Web Site: https://search.jamas.or.jp/link/ui/2005050496
• Text Lang: en
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed