Dentin Regeneration and Reconstruction: Comparison of in vivo vs. in vitro Dentin Bonds with Self-etching/Self-priming Adhesive Systems

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The use of dog teeth <i>in vivo</i> provides a useful screening model between <i>in vitro</i> tests and human clinical trials. In this experiment, the effects of post-insertion time on the structure and strength of resin-dentin bonds were evaluated. The purpose of this experiment was to determine the resin-dentin bond strengths in dog teeth <i>in vitro</i> vs. <i>in vivo</i> (immediate, 3 weeks and 3 months after restoration ), and to investigate the resin-dentin interfaces with scanning electron microscopy ( SEM ). Box-form Class V cavities were prepared into labial middle coronal dentin in 4 anesthetized beagle dogs. The teeth were bonded with Fluoro Bond ( FB, Shofu ) or Clearfil Liner Bond 2 ( LB, Kuraray ) and restored with Clearfil AP-X resin composite ( Kuraray ). The teeth were surgically removed immediately, 3 weeks or 3 months after restoration, serially sliced and trimmed to a surface area of 1mm<sup>2</sup> for microtensile bond strength test (MTBS). For the <i>in vitro</i> study, extracted dog teeth were used. <i>In vitro</i> and <i>in vivo</i> immediate bond strengths of both adhesive systems were 33.7 to 36.3 MPa, which were not significantly different. <i>In vivo</i> bond strengths of both adhesive systems at 3 weeks and 3 months were significantly lower than those measured immediately <i>in vitro</i> or <i>in vivo</i>. SEM showed the formation of hybrid layers with both adhesive systems when tested immediately <i>in vitro</i> or <i>in vivo</i>, but after 3 month <i>in vivo</i>, hybrid layers could not clearly seen. The fall in bond strengths noted with time may have been due to occlusal stress, and the influence of oral or dentinal fluids on the morphology of the hybrid layers and resin tags.


  • Journal of Oral Tissue Engineering

    Journal of Oral Tissue Engineering 1(1), 80-88, 2004

    Japanese Association of Regenerative Dentistry


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