Comparison of FGF1 (aFGF) Expression between the Dorsal Motor Nucleus of Vagus and the Hypoglossal Nucleus of Rat

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  • Toyoda Ken-ichiro
    Molecular Neuroscience Research Center, Shiga University of Medical Science Department of Otolaryngology-Head and Neck Surgery, Kyoto Prefectural University of Medicine
  • Okano Hiroyuki
    Molecular Neuroscience Research Center, Shiga University of Medical Science Department of Otolaryngology-Head and Neck Surgery, Kyoto Prefectural University of Medicine
  • Bamba Hitoshi
    Department of Otolaryngology-Head and Neck Surgery, Kyoto Prefectural University of Medicine
  • Hisa Yasuo
    Department of Otolaryngology-Head and Neck Surgery, Kyoto Prefectural University of Medicine
  • Oomura Yutaka
    Department of Physiology, School of Medicine, Kyushu University
  • Imamura Toru
    Signaling Molecules Research Laboratory, National Institute of Advanced Industrial Science and Technology (AIST)
  • Furukawa Shoei
    Laboratory of Molecular Biology, Gifu Pharmaceutical University
  • Tooyama Ikuo
    Molecular Neuroscience Research Center, Shiga University of Medical Science

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Neurons in the dorsal motor nucleus of the vagus (DMNV) are more severely affected by axonal injury than most other nerves, such as those of the hypoglossal nucleus. However, the mechanism underlying such a response remains unclear. In this study, we compared the expression of fibroblast growth factor 1 (FGF1), a neurotrophic factor, between the DMNV and the hypoglossal nucleus by RT-PCR and immunohistochemical analyses. RT-PCR showed that the level of FGF1 mRNA expression in the DMNV was lower than that in the hypoglossal nucleus (P<0.01). Immunohistochemistry revealed that FGF1 was localized to neurons. FGF1-positive neurons in large numbers were evenly distributed in the hypoglossal nucleus, whereas FGF1-positive neurons were located in the lateral part of the DMNV. Double immunostaining for FGF1 and choline acetyltransferase demonstrated that 22.7% and 78% of cholinergic neurons were positive for FGF1 in the DMNV and hypoglossal nucleus, respectively. A tracing study with cholera toxin B subunit (CTb) demonstrated that cholinergic neurons sending their axons from the DMNV to the superior laryngeal nerve were FGF1-negative. The results suggest that the low expression of FGF1 in the DMNV is due to severe damage of neurons in the DMNV.<br>

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