Inhibitory effect of constituents of Bu-Shen-Jian-Gu-Tang on osteoclast-like cell formation

• SHI Liying

  Institute of Natural Medicine, University of Toyama

• TEZUKA Yasuhiro

  Institute of Natural Medicine, University of Toyama

• MIYAHARA Tatsuro

  Faculty of Medicine, University of Toyam

• YIN Jun

  School of Traditional Chinese Medicines, Shenyang Pharmaceutical University

• NOBUKAWA Takahiro

  Kanazawa Medical University

• KADOTA Shigetoshi

  Institute of Natural Medicine, University of Toyama

抄録

The constituents of Bu-Shen-Jian-Gu-Tang and their inhibitory activities on osteoclast-like cell formation were investigated. Bu-Shen-Jian-Gu-Tang was fractionated by a Diaion HP-20 column with EtOH-H₂O, and the 60% and 90% EtOH-H₂O fractions showed more potent inhibitory activities on osteoclast-like cell formation and bone resorption. Chemical investigation of these two fractions resulted in the isolation of 11 compounds, for which the structures were elucidated based on spectroscopic analysis and chemical reactions. The inhibitory activities of the isolated compounds on osteoclast-like cell formation were evaluated. Among the eleven compounds, icariin (1), icarisid II (2), 2"-O-rhamnosylicariside II (3), kaempferol (8), chikusetsusaponin IVa (10) and chikusetsusaponin V (11) showed potent inhibitory activities at a concentration of 2 μM without toxic effects. The preventive effect of Bu-Shen-Jian-Gu-Tang on bone loss may be due to the inhibitory activities of these compounds.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｔｒａｄｉｔｉｏｎａｌ　Ｍｅｄｉｃｉｎｅｓ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｔｒａｄｉｔｉｏｎａｌ　Ｍｅｄｉｃｉｎｅｓ 24 (1), 19-23, 2007

  和漢医薬学会

キーワード

• antiosteoporotic activity
• Kampo formula
• Bu-Shen-Jian-Gu-Tang
• osteoclast
• inhibitory activity

詳細情報

• CRID: 1390001205233153024
• NII論文ID: 110006224306
• NII書誌ID: AA12035198
• DOI: 10.11339/jtm.24.19
• ISSN: 18813747
• ISSN: 18801447
• HANDLE: 10110/1992
• NDL書誌ID: 8743224
• Web Site: https://toyama.repo.nii.ac.jp/records/5331
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8743224
• Web Site: https://search.jamas.or.jp/link/ui/2007205015
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可