L-Type Amino Acid Transporter-1 Expressed in Human Astrocytomas, U343MGa

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  • Asano Shinji
    Department of Molecular Physiology, College of Information Science and Engineering, Ritsumeikan University
  • Kameyama Megumi
    Graduate School of Medical and Pharmaceutical Sciences, University of Toyama
  • Oura Ayako
    Department of Molecular Physiology, College of Information Science and Engineering, Ritsumeikan University
  • Morisato Anna
    Department of Molecular Physiology, College of Information Science and Engineering, Ritsumeikan University
  • Sakai Hideki
    Graduate School of Medical and Pharmaceutical Sciences, University of Toyama
  • Tabuchi Yoshiaki
    Life Science Research Center, University of Toyama
  • Chairoungdua Arthit
    Department of Pharmacology and Toxicology, Kyorin University School of Medicine
  • Endou Hitoshi
    Department of Pharmacology and Toxicology, Kyorin University School of Medicine
  • Kanai Yoshikatsu
    Department of Pharmacology and Toxicology, Kyorin University School of Medicine

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Abstract

LAT1 (L-type amino acid transporter 1), one of the L-type amino acid transporters, transports the branched and aromatic amino acids. LAT1 requires the heavy chain of 4F2 antigen (4F2hc) for the functional expression as an amino acid transporter. The expression of this transporter is up-regulated in tumor cells and rapidly-growing cells to support their proliferation. Here, we studied the expression of LAT1 and 4F2hc in human cultured cells by real-time PCR and Western blot, and found that human brain astrocytomas, U343MGa, highly expressed LAT1 and 4F2hc mRNAs and proteins. The uptake of [14C]leucine by U343MGa cells is Na+-independent and inhibited by BCH (2-amino-2-norbornane carboxylic acid), and branched and aromatic amino acids, indicating that the LAT1 is expressed at the cell surface. Pulse chase labeling and surface labeling experiments of this cell line indicate that the protein synthesis of LAT1 and 4F2hc is slow, however, the heterodimeric complex assembled in the cells is very stable, and that the disulfide bond between the LAT1 and 4F2hc is not directly involved in the stability of the heterodimer.

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