A Taxon-specific Oligonucleotide Primer Set for PCR-based Detection of Soil Ciliate

• Puitika Tunjung

  College of Agriculture, Ibaraki University

• Kasahara Yasuhiro

  College of Agriculture, Ibaraki University Institute of Low Temperature Science, Hokkaido University

• Miyoshi Norikazu

  Department of Upland Farming, National Agricultural Research Center for Tohoku Region

• Sato Yoshinori

  College of Agriculture, Ibaraki University

• Shimano Satoshi

  Department of Upland Farming, National Agricultural Research Center for Tohoku Region Miyagi University of Education

Abstract

A PCR primer set was designed to amplify the 18S rRNA gene from the phylum Ciliophora (ciliates). The pair amplified DNA from 5 organisms representing the major taxonomic groups of ciliates but not from 13 non-ciliate organisms. To test the ability of the primers to amplify ciliate rRNA genes from environmental samples, a clone library was constructed from an agricultural soil sample and analyzed. The rRNA gene sequences of all clones were affiliated with the Ciliophora. This newly developed PCR primer set is able to amplify ciliate rRNA genes from soil, and may be useful for the detection and identification of ciliates.<br>

Journal

• Microbes and Environments

  Microbes and Environments 22 (1), 78-81, 2007

  Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

Keywords

• 18S rRNA gene
• ciliate
• PCR

Details

• CRID: 1390001204345220224
• NII Article ID: 110006242034
• NII Book ID: AA11551577
• DOI: 10.1264/jsme2.22.78
• ISSN: 13474405
• ISSN: 13426311
• NDL BIB ID: 8691987
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8691987
• Web Site: http://www.jstage.jst.go.jp/article/jsme2/22/1/22_1_78/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed