The Influence of Saliva on Initial Formation of Oral Biofilm : Assessment by a Flow-cell System in vitro

• 田村 光平

Bibliographic Information

Other Title

• 口腔細菌バイオフィルム初期形成に及ぼす唾液の影響
• 口腔細菌バイオフィルム初期形成に及ぼす唾液の影響--フローセルシステムによるin vitro評価
• コウクウ サイキン バイオフィルム ショキ ケイセイ ニ オヨボス ダエキ ノ エイキョウ フローセル システム ニ ヨル in vitro ヒョウカ
• ― フローセルシステムによるin vitro評価 ―

Abstract

Recently, the influences of biofilm have been focused on as serious problems in various fields such as biomaterial sciences and environmental engineering. Oral biofilm, generally called "dental plaque", is also regarded as a direct causative pathogen of dental caries and periodontal disease in dentistry. Therefore, it is indispensable to control biofilm for the prevention and improvement of oral diseases. In this study, a parallel plate flow-cell system using automated image analysis with a new algorithm was developed to enumerate initial biofilm formation. The aim of this study was to observe the properties and capabilities of the flow-cell system using human saliva-induced bacterial cells. In addition, the effects of salivary treatment of bacterial cells and the substratum (slide glass) on bacterial deposition were examined in consideration of the clinical oral state. It was found that the flow-cell system was useful to quantify initial bacterial deposition in real time. Five repetitions of each experiment indicated that the error levels (coefficient of variation) of the initial deposition rate J_0 values were within 24.6% for untreated glass plates, 34.5% for saliva-treated glass plates, 17.4% for saliva-treated cells, and 31.9% for saliva-treated glass plates and cells. It was shown that the initial adhesion of bacterial cells decreased specifically by saliva treatment of glass plates (t-test : p<0.05). In contrast, it was indicated that the initial adhesion of bacterial cells increased remarkably by saliva treatment of bacterial cells (t-test : p< 0.005). It was concluded that initial biofilm formation using human saliva-induced bacterial cells can be examined quantitatively by the parameter J_0 derived from the flowcell system of this study, and that saliva treatment of bacterial cells and the substratum strongly influences initial biofilm formation.

Journal

• Dental Journal of Iwate Medical University

  Dental Journal of Iwate Medical University 32 (2), 136-138, 2007

  The Dental Society of Iwate Medical University

Keywords

• Flow-cell
• Oral bacteria
• Biofilm
• Saliva

Details

• CRID: 1390282679567068416
• NII Article ID: 110006368264
• NII Article ID: 110006249473
• NII Book ID: AN00081407
• DOI: 10.20663/iwateshigakukaishi.32.2_136
• DOI: 10.5834/jdh.57.2_102
• ISSN: 24241822
• ISSN: 21897379
• ISSN: 03851311
• ISSN: 00232831
• NDL BIB ID: 8819513
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8819513
• Web Site: https://search.jamas.or.jp/link/ui/2007210651
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed