Monitoring of DNA complex formation by means of quartz crystal microbalance

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Author(s)

    • HAYAKAWA T
    • Department of Dental Biomaterials, Nihon University School of Dentistry at Matsudo
    • FUKUSHIMA Tadao
    • Department of Dental Engineering, Bioengineering Section, Fukuoka Dental College
    • KAWAGUCHI Minoru
    • Department of Dental Engineering, Bioengineering Section, Fukuoka Dental College
    • TANIGUCHI Kunihisa
    • Department of Morphological Biology, Section of Pathology, Fukuoka Dental College
    • KAMINISHI Hidenori
    • Department of Functional Bioscience, Section of Infection Biology, Fukuoka Dental College

Abstract

The double-helical structure of double-stranded DNA enables it to form complexes with antibiotics and cytokines. The present study investigated the complex formation for antibiotic or cytokine towards DNA using quarts-crystal microbalance (QCM) technique. The resonance frequency decreases in QCM system linearly upon the increase of adsorbed mass on the QCM sensor at the nanogram level. Biotinylated DNA can easily bind avidin molecules, and DNA could be immobilized onto QCM sensor, which was sputtered with gold. The crystal resonant frequency shift was immediately measured after the injection of daunorubicin hydrochloride, which was an antibiotics, and of basic fibroblast growth factor (b-FGF), which was a growth factor. The present study can monitor the complex formation between daunorubicin hydrochloride or b-FGF and DNA using QCM method, and binding amount of daunorubicin hydrochloride or b-FGF towards DNA can be easily estimated by the frequency decrease. In addition, the difference of binding mode of daunorubicin hydrochloride and b-FGF towards DNA was revealed by the frequency shift. The QCM method is a useful for monitoring DNA complex formation with antibiotics or cytokines.

Journal

  • J Oral Tissue Engin

    J Oral Tissue Engin 4, 61-67, 2006

    Japanese Association of Regenerative Dentistry

Cited by:  1

Codes

  • NII Article ID (NAID)
    110006263698
  • NII NACSIS-CAT ID (NCID)
    AA1195240X
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13489623
  • Data Source
    CJPref  NII-ELS 
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