Direct Profiling of rRNA in Saline Wastewater Treatment Samples Using an Oligonucleotide Microarray

Access this Article

Search this Article

Author(s)

Abstract

Fifteen oligonucleotide probes, targeting a broad range of microorganisms, were spotted on a DNA microarray. Specificity and reproducibility were evaluated using fluorescently labeled rRNA from 16 bacterial and eukaryal strains. The oligonucleotide microarray was then used to analyze activated sludge samples of saline industrial wastewater in which microbial communities had been characterized previously using 16S rRNA clone libraries. The results obtained were partially consistent with the results of cloning, and demonstrate the possibility of using oligonucleotide microarrays for the monitoring and characterization of saline industrial wastewater populations.<br>

Journal

  • Microbes and Environments

    Microbes and Environments 22(2), 116-122, 2007-06-01

    Japanese Society of Microbial Ecology

References:  25

Cited by:  2

Codes

  • NII Article ID (NAID)
    110006290373
  • NII NACSIS-CAT ID (NCID)
    AA11173196
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13426311
  • NDL Article ID
    8765490
  • NDL Source Classification
    ZR5(科学技術--生物学--菌類・微生物)
  • NDL Call No.
    Z54-J644
  • Data Source
    CJP  CJPref  NDL  NII-ELS  J-STAGE 
Page Top