Direct Profiling of rRNA in Saline Wastewater Treatment Samples Using an Oligonucleotide Microarray

• Urakawa Hidetoshi

  Ocean Research Institute, The University of Tokyo

• Matsumoto Junpei

  Department of Chemical Engineering, Waseda University

• Hoshino Tatsuhiko

  Department of Chemical Engineering, Waseda University

• Tsuneda Satoshi

  Department of Chemical Engineering, Waseda University

Abstract

Fifteen oligonucleotide probes, targeting a broad range of microorganisms, were spotted on a DNA microarray. Specificity and reproducibility were evaluated using fluorescently labeled rRNA from 16 bacterial and eukaryal strains. The oligonucleotide microarray was then used to analyze activated sludge samples of saline industrial wastewater in which microbial communities had been characterized previously using 16S rRNA clone libraries. The results obtained were partially consistent with the results of cloning, and demonstrate the possibility of using oligonucleotide microarrays for the monitoring and characterization of saline industrial wastewater populations.<br>

Journal

• Microbes and Environments

  Microbes and Environments 22 (2), 116-122, 2007

  Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

Keywords

• oligonucleotide microarray
• saline industrial wastewater
• ribosomal RNA

Details

• CRID: 1390282679322479232
• NII Article ID: 110006290373
• NII Book ID: AA11551577
• DOI: 10.1264/jsme2.22.116
• ISSN: 13474405
• ISSN: 13426311
• NDL BIB ID: 8765490
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8765490
• Web Site: http://www.jstage.jst.go.jp/article/jsme2/22/2/22_2_116/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed