Preparation and Stability of Avidin-Modified Glass Slips

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  • SATO Masatoshi
    Department of Chemistry, College of Humanities and Sciences, Nihon University
  • YANAGIDA Koji
    Department of Chemistry, College of Humanities and Sciences, Nihon University
  • TANAKA Ayako
    Department of Chemistry, College of Humanities and Sciences, Nihon University
  • SUGAWARA Masao
    Department of Chemistry, College of Humanities and Sciences, Nihon University

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Other Title
  • アビジン修飾ガラス基板の作製と安定性の比較
  • アビジン シュウショク ガラス キバン ノ サクセイ ト アンテイセイ ノ ヒカク

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Abstract

The formation of avidin sites on the surface of glass slips by chemical modification and an avidin-biotin technique was investigated. In a chemical modification approach, a glass surface was treated with 3-mercaptopropyltrimethoxysilane, on which avidin was introduced by an amine coupling method. In the avidin-biotin technique, biotin sites were formed on glass slips by the patterning of biotin-4-fluorescein on bovine serum albumin (BSA)-modified and BSA-adsorbed glass slips by UV irradiation. Avidin-modified glass slips were prepared by treating the biotin sites with an avidin solution. The avidin slips prepared by UV patterning were able to be kept in the dark at 4°C for at least 5 days, while the chemically modified slips were able to be kept for one day. The preparation of avidin arrays by UV patterning was also demonstrated. The avidin slips appeared to be useful for introducing biomolecules to design a variety of sensing systems.<br>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 56 (6), 485-489, 2007

    The Japan Society for Analytical Chemistry

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