Genetic Variation in Lipoxygenase Activity Among Japanese Malting Barley Cultivars and Identification of a New Lipoxygenase-1 Deficient Mutant

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  • Oozeki Mika
    National designed research units for malting barley quality improvement and for two-row barley breeding, Tochigi Prefectural Agricultural Experiment Station
  • Nagamine Takashi
    National designed research units for malting barley quality improvement and for two-row barley breeding, Tochigi Prefectural Agricultural Experiment Station
  • M. Ikeda Tatsuya.
    Western National Agricultural Research Center
  • Suzuki Yasuhiro
    Crop Research Institute
  • Sekiwa Takahiro
    National designed research units for malting barley quality improvement and for two-row barley breeding, Tochigi Prefectural Agricultural Experiment Station Present address: Tochigi Prefectural Sustainable Agriculture Extension Center
  • Yamaguchi Emiko
    National designed research units for malting barley quality improvement and for two-row barley breeding, Tochigi Prefectural Agricultural Experiment Station Present address: Shimotsuga Agricultural Extension Office
  • Kato Tsuneo
    National designed research units for malting barley quality improvement and for two-row barley breeding, Tochigi Prefectural Agricultural Experiment Station

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Other Title
  • 我が国のビール大麦品種におけるリポキシゲナーゼ活性の変異と新たな活性欠失突然変異系統の作出
  • ワガクニ ノ ビール オオムギ ヒンシュ ニ オケル リポキシゲナーゼ カッセイ ノ ヘンイ ト アラタナ カッセイ ケッシツ トツゼン ヘンイ ケイトウ ノ サクシュツ

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Abstract

We studied the genetic variation in barley grain lipoxygenase (LOX) activity, developed a simple assay to estimate the LOX activity, and identified a new LOX-deficient mutant line. Grain LOX activity differed considerably by as much as 2.2-fold among 72 Japanese cultivars. Most of the cultivars with a high LOX activity had been released in the past, such as “Tochigi golden melon”. We developed a simple assay to estimate the barley LOX activity by modifying the soybean LOX isozyme detection system. By using this assay, we identified a LOX-deficient mutant line “Daikei LM1” from the 258 M3 lines derived from the sodium azide-treated, 6-row malting cultivar “Karl.” DNA sequence analysis of the Lox-1 gene of “Daikei LM1” revealed a single nucleotide substitution (C to T) at nucleotide 2005, which introduced an internal stop codon into the third exon of Lox-1.<br>

Journal

  • Breeding Research

    Breeding Research 9 (2), 55-61, 2007

    Japanese Society of Breeding

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