Expression Patterns of the BRCA1 Splicing Variants in Canine Normal Tissues and Mammary Gland Tumors

• SUGIURA Takaaki

  Laboratory of Veterinary Radiology, Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• MATSUYAMA Satoshi

  Laboratory of Veterinary Radiology, Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• AKIYOSI Hideo

  Laboratory of Advanced Diagnosis and Treatment, Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• TAKENAKA Shigeo

  Laboratory of Molecular Biology, Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• YAMATE Jyoji

  Laboratory of Veterinary Pathology, Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• KUWAMURA Mitsuru

  Laboratory of Veterinary Pathology, Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• AOKI Mica

  Laboratory of Veterinary Surgery, Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• SHIMADA Terumasa

  Laboratory of Veterinary Surgery, Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• OHASHI Fumihito

  Laboratory of Veterinary Surgery, Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• KUBO Kihei

  Laboratory of Veterinary Radiology, Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University

Bibliographic Information

Other Title

• 犬正常組織と乳腺腫瘍におけるBRCA1スプライシングバリアントmRNAの発現パターン

Abstract

Human BRCA1 is familial breast cancer susceptibility gene. Recently, decreased BRCA1 mRNA and protein expression has been identified in sporadic breast tumors. In the reported human BRCA1 splicing variants, delta11b lacks the majority of exon11 and is suspected to have a distinct function in normal tissues. The splicing variants display a variety of expression pattern in breast cancer samples. Although mammary gland tumor is important disease in dog, there are few reports for BRCA1 in the canine tumors. In this study, we examined the relative amounts of BRCA1 splicing variants mRNA in canine normal and mammary tumor samples by RT-PCR to investigate whether there is the altered expression of variant mRNAs in the canine tumor as reported in human. The exon11b-defecting RT-PCR products were observed in all the normal tissues examined and the nucleotide sequence was quite similar to that of human BRCA1 delta11b. In some tumor samples, we did not detect the products targeted for exon10-13 and exon14-15, while these products were observed in all the normal samples examined. Especially, the relative amounts of the exon11-defecting products were remarkably decreased in most of the tumors (11/16).<br>

Journal

• Journal of Veterinary Medical Science

  Journal of Veterinary Medical Science 69 (6), 587-592, 2007

  JAPANESE SOCIETY OF VETERINARY SCIENCE

Keywords

• BRCA1
• canine
• mammary tumor
• RT-PCR
• splicing variant

Details

• CRID: 1390001206430202368
• NII Article ID: 110006318093
• NII Book ID: AA10796138
• DOI: 10.1292/jvms.69.587
• ISSN: 13477439
• ISSN: 09167250
• NDL BIB ID: 8871105
• Web Site: https://agriknowledge.affrc.go.jp/RN/2030742625
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8871105
• Web Site: http://www.jstage.jst.go.jp/article/jvms/69/6/69_6_587/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2007228045
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed