Soluble cytochromes c of a KCN-resistant mutant of an obligate methylotroph, Methylomonas sp.

• TANI Yoshiki

  Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University

• YOON Byung Dae

  Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University

• YAMADA Hideaki

  Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University

Abstract

A KCN-resistant mutant, Methylomonas sp. YK 56, contained three kinds of soluble cytochromes c (cytochromes c-I, c-II, and c-III) though the wild type strain contained two kinds (cytochromes c-I and c-III). The proportion of the three cytochromes c of the mutant were 2.4, 71.5, and 26.1%, and that of the two cytochromes c of the wild type strain were 2.1 and 97.9%, respectively.<br> Cytochromes c-II and c-III of the mutant were purified by a procedure involving ammonium sulfate fractionation and DEAE-, CM-cellulose, and Sephadex G-150 column chromatography. Cytochrome c-II was obtained as crystals with ammonium sulfate. Both absorption peaks of the α-band of the two cytochromes c were at 551.5 nm at room temperature and the β-band of cytochrome c-II had a shoulder at 530 nm. Molecular weights of the two cytochromes c were 16, 000 and 20, 000, respectively and their isoelectric points were 4.1 and 3.5, respectively.

Journal

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 50 (10), 2545-2552, 1986

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

Details

• CRID: 1390001206463545856
• NII Article ID: 110006322728
• NII Book ID: AA00515312
• DOI: 10.1271/bbb1961.50.2545
• COI: 1:CAS:528:DyaL2sXhvF2i
• ISSN: 18811280
• ISSN: 00021369
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed