Interaction of proteinaceous protease inhibitor of Bacillus subtilis with intracellular proteases from the same strain.

• NISHINO Toyokazu

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture

• MURAO Sawao

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture Present address: Kumamoto Institute of Technology

抄録

Intracellular protease inhibitor of B. subtilis IFO 3027 inhibits cytoplasmic serine proteases (IP-I, IP-II and IP-Ill), but it does not affect membrane-bound serine protease from the same strain. Inhibitory equivalent and binding titration studies revealed that the inhibitor binds and inhibits one mol of IP-I (a major protease in the cells). The inhibitor constant, Ki, against IP-I is estimated to be approximately 10^-9 M. The inhibitor binds and inhibits IP-II and IP-Ill in the same manner as IP-I, but its association with these enzymes is strongly repressed by the substrate (casein). Incubation of the inhibitor with membrane-bound protease results in the loss of the inhibitory activity.

収録刊行物

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 50 (12), 3065-3070, 1986

  公益社団法人 日本農芸化学会

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1390001206464435072
• NII論文ID: 110006322824
• NII書誌ID: AA00515312
• DOI: 10.1271/bbb1961.50.3065
• ISSN: 18811280
• ISSN: 00021369
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可