High-performance liquid chromatographic measurement of nicotinamide N-oxide in urine after extracting with chloroform.

• SHIBATA Katsumi

  Department of Food Science and Nutrition, Teikoku Women's University

抄録

The measurement of nicotinamide N-oxide by high-performance liquid chromatography is described. The method used a Chemcosorb 5-ODS-H (150 × 4.5 mm i.d., particle size 5 μm) column eluted with 10 mM potassium dihydrogenphosphate (pH was adjusted to 3.0 by adding concentrated phosphoric acid) containing 0.1 g/l of sodium 1-octanesulfonate-methanol (100:4, v/v) at a flow-rate of 1.0ml/min. The UV detector was set at 260nm. The detection limit for nicotinamide N-oxide was 2pmol (276 pg) at a signal-to-noise ratio of 5:1. Under these conditions, nicotinamide N-oxide was eluted at about 3.2 min. This technique was used to analyze rat urine following effective clean-up steps with chloroform, the total analysis time being about 13 min.

収録刊行物

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 53 (5), 1329-1331, 1989

  公益社団法人 日本農芸化学会

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1390282681442095872
• NII論文ID: 110006324319
• NII書誌ID: AA00515312
• DOI: 10.1271/bbb1961.53.1329
• COI: 1:CAS:528:DyaL1MXktlKjt7Y%3D
• ISSN: 18811280
• ISSN: 00021369
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可