Purification and characterization of multiple carboxymethyl cellulases from bacillus sp. KSM-522.

• OKOSHI Hiromi

  Tochigi Research Laboratories of Kao Corporation

• OZAKI Katsuya

  Tochigi Research Laboratories of Kao Corporation

• SHDCATA Shitsuw

  Tochigi Research Laboratories of Kao Corporation

• OSHINO Kazushi

  Tochigi Research Laboratories of Kao Corporation

• KAWAI Shuji

  Tochigi Research Laboratories of Kao Corporation

• ITO Susumu

  Tochigi Research Laboratories of Kao Corporation

Abstract

Three endo-β-1, 4-glucanases (CMCases E-I, E-II and E-III) from Bacillus sp. KSM-522 were purified to homogeneity by repeated chromatography on DEAE Bio-Gel A. The molecular weights were 78, 000 for E-I, and 61, 000 for both E-II and E-III, as judged on sodium dodecyl sulfate polyacrylamide gel electrophoresis. The optimum pH values for activity and isoelectric points were around 6 and 4.4 for E-I, and 7-10 and 3.5 for both E-II and E-III, respectively. These CMCases hydrolyzed CMC in a similar random fashion. The alkaline CMCases, E-II and E-III, were not distinguishable from each other with respect to temperature-activity curves, temperature-stability curves, substrate specificity, patterns of action on cellooligosaccharides, susceptibility to inhibitors or effects of metal ions.

Journal

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 54 (1), 83-89, 1990

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

Details

• CRID: 1390001206463467136
• NII Article ID: 110006324553
• NII Book ID: AA00515312
• DOI: 10.1271/bbb1961.54.83
• COI: 1:CAS:528:DyaK3cXhtlCqurc%3D
• ISSN: 18811280
• ISSN: 00021369
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed