Steady-state near-infrared detection of singlet molecular oxygen: A stren-volmer quenching experiment with luminol, superoxide dismutase, and Cypridina luciferin analogues.

• SUZUKI Nobutaka

  Biophoton Project, Research Development Corporation of Japan (JRDC)

• MIZUMOTO Iwao

  Research Institute of Electrical Communication, Tohoku University

• TOYA Yoshiaki

  Department of Chemistry, Aichi University of Education

• NOMOTO Tateo

  Department of Chemistry, Faculty of Education, Mie University

• MASHIKO Shinro

  Communications Research Laboratory, Ministry of Posts and Telecommunications

• INABA Humio

  Biophoton Project, Research Development Corporation of Japan (JRDC) Research Institute of Electrical Communication, Tohoku University

Abstract

A sensitive near-infrared detection system with improvements was used to study the quenching of the steady-estate luminescence of singlet molecular oxygen at 1270 nm. Stern-Vohner plots which were linear up to 80% quenching by luminol of the ¹O₂ generated by rose bengal yielded rate constants of 9.30 × 10⁸ and 1.40 × 10⁹ M^-1 sec^-1 for the quenching of ¹Q₂ in pH 7.1 and pH 10.1 buffer solutions (25 HIM). Quenching rate constants of ¹O₂ by superoxide dismutase and Cypridina luciferin analogues, 2-methyl-6-phenyl- and 2-methyl-6-[p-[2-[sodium 3-carboxylato-4-(6-hydroxy-3-xanthenon-9-yl)-phenylthioureylene]ethyleneoxy]phenyl]-3, 7-dihydroimidazo[1, 2-a]pyrazin-3-one (CLA and FCLA) were measured similarly as 2.73 × 10⁹, 6.30 × 10⁸ and 8.00 × 10⁸ M^-1 sec^-1 in the pH 7.1 buffer solutions, respectively.

Journal

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 54 (11), 2783-2787, 1990

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

Details

• CRID: 1390282681439885952
• NII Article ID: 110006324650
• NII Book ID: AA00515312
• DOI: 10.1271/bbb1961.54.2783
• COI: 1:CAS:528:DyaK3MXnvVSnsQ%3D%3D
• ISSN: 18811280
• ISSN: 00021369
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed