Cloning and expression in Escherichia coli of Clostridium stercorarium strain F-9 genes related to xylan hydrolysis.

• SAKKA Kazuo

  Department of Bioscience, Faculty of Bioresources, Mie University

• KOJIMA Yuzo

  Department of Bioscience, Faculty of Bioresources, Mie University

• YOSHIKAWA Kazuhiro

  Department of Bioscience, Faculty of Bioresources, Mie University

• SHIMADA Kyo

  Department of Bioscience, Faculty of Bioresources, Mie University

抄録

Nine distinct fragments of Clostridium stercorarium strain F-9 (which was isolated and identified in this laboratory) DNA have been cloned in Escherichia coli and shown to express enzymatic activities related to xylan hydrolysis. Two of the 9 E. coli clones showed xylanase activity, one showed β-xylosidase activity as well as xylanase activity, and the other 6 showed β-xylosidase activity. On comparing the restriction maps of the xylanase and xylosidase genes cloned in this study with those reported so far, it was concluded that all the 9 clones carried hitherto unidentified genes. All the enzymes expressed in the E. coli clones were thermophilic.

収録刊行物

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 54 (2), 337-342, 1990

  公益社団法人 日本農芸化学会

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1390001206460209792
• NII論文ID: 110006324765
• NII書誌ID: AA00515312
• DOI: 10.1271/bbb1961.54.337
• COI: 1:CAS:528:DyaK3cXhslemt7g%3D
• ISSN: 18811280
• ISSN: 00021369
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可