Biochemical mechanism of C-P bond formation of bialaphos: Use of gene manipulation for the analysis of the C-P bond formation step.

• HIDAKA Tomomi

  Institute of Applied Microbiology, The University of Tokyo

• KARA Osamu

  Pharmaceutical Research Center, Meiji Seika Kaisha, Ltd.

• IMAI Satoshi

  Pharmaceutical Research Center, Meiji Seika Kaisha, Ltd.

• ANZAI Hiroyuki

  Pharmaceutical Research Center, Meiji Seika Kaisha, Ltd.

• MURAKAMI Takeshi

  Pharmaceutical Technology Laboratory, Meiji Seika Kaisha, Ltd.

• NAGAOKA Kozo

  Pharmaceutical Research Center, Meiji Seika Kaisha, Ltd.

• SETO Haruo

  Institute of Applied Microbiology, The University of Tokyo

Abstract

One of the three C-P bond formation steps, defined as step 5 in the bialaphos (BA) biosynthetic pathway, was analyzed using a new BA non-producing mutant NP71. The mutant was derived from a BA producer by gene replacement of an unidentified region next to the gene responsible for the step 5 deficiency of the mutant NP213, obtained by conventional mutation procedures. Biochemical analysis of these two mutants indicated that NP71 was defective in the formation of carboxyphosphonoenolpyruvate (CPEP), while NP213 lacked the enzyme CPEP phosphonomutase, which catalyzed the intramolecular rearrangement of CPEP.

Journal

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 54 (8), 2121-2125, 1990

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

Details

• CRID: 1390282681439590912
• NII Article ID: 110006325085
• NII Book ID: AA00515312
• DOI: 10.1271/bbb1961.54.2121
• COI: 1:CAS:528:DyaK3cXlvVOktbY%3D
• ISSN: 18811280
• ISSN: 00021369
• PubMed: 1368613
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed