E-15, a Novel Polysaccharide Mitogen from Nocardia Specific for B Cells.

• KURISAKI Tomohiro

  Department of Agricultural Chemistry, The University of Tokyo

• MUNEMURA Kazuko

  Department of Agricultural Chemistry, The University of Tokyo

• KOBAYASHI Kyuichi

  Department of Agricultural Chemistry, The University of Tokyo

• YAMAMOTO Masakuni

  Department of Agricultural Chemistry, The University of Tokyo

• HAYASHI Midori

  Department of Agricultural Chemistry, The University of Tokyo

• NAKAMURA Akito

  Department of Agricultural Chemistry, The University of Tokyo

• MAGAE Junji

  Department of Bioengineering, Tokyo Institute of Technology

• KUSAKABE Hiroo

  RIKEN Institute

• URAMOTO Masakazu

  RIKEN Institute

• ISONO Kiyoshi

  RIKEN Institute

• NAGAI Kazuo

  Department of Bioengineering, Tokyo Institute of Technology

• YAMASAKI Makari

  Department of Agricultural Chemistry, The University of Tokyo

抄録

A novel mitogen, E-15, which induced blastogenesis of mouse splenocytes, was purified from the culture filtrate of an actinomycete through ethanol precipitation, anion and cation exchange column chromatography, and gel filtration. The producing organism was identified as Nocardia asteroides. Spectronic study demonstrated that it was a polysaccharide. Acid hydrolysis of E-15 yielded glucose and glucosamine. The active substance was eluted at the molecular mass between 90 kDa and 750 kDa on gel filtration.<br> E-15 induced a mitogenic response of mouse splenocytes above 1 μg/ml and its potency of induction was superior to a lipopolysaccharide that is known to be a polyclonal B cell-specific mitogen. It showed no toxicity up to 100μg/ml. The cell surface phenotypes of the blast cells induced by E-15 were analyzed by flow cytometry; they had surface immunoglobulins but no Lyt-2 antigen. Thus it was suggested that E-15 was a B-cell-specific mitogen.

収録刊行物

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 55 (12), 2987-2991, 1991

  公益社団法人 日本農芸化学会

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1390001206464306176
• NII論文ID: 110006325320
• NII書誌ID: AA00515312
• DOI: 10.1271/bbb1961.55.2987
• ISSN: 18811280
• ISSN: 00021369
• Web Site: https://search.jamas.or.jp/link/ui/1993202171
• 本文言語コード: en
• データソース種別:
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• 抄録ライセンスフラグ: 使用不可