紫外線照射がヘアレスマウスの免疫器官および内分泌器官に及ぼす影響 Effects of ultraviolet irradiation on the histological changes in immune and endocrine organs of hairless mice

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Abstract

紫外線が皮膚に及ぼす影響については、多くの研究がある。しかしながら、皮膚以外に及ぼす影響についての研究は少ない。そこで、本研究においては、ヘアレスマウスを用いて免疫器官および内分泌器官に及ぼす影響について組織学的に検討した。雌のヘアレスマウス(Hr-/Kud;8週令)を材料として用いた。紫外線を含む太陽光を模した人工太陽光ランプ(VITA-LITE)を光源として用いた。また、紫外線を除去するために紫外線カットフィルム(アクリプレン;三菱レイヨン)を用いた。明暗周期(12時間明:12時間暗)、恒温(25℃)の条件下で動物を飼育した。紫外線を含む人工太陽光に照射されたマウスは、アクリプレンフィルターで紫外線をカットしたマウスに比べてシクロブタン型ピリミジンダイマーが顕著に多かった。器官重量(胸腺、脾臓、卵巣、肝臓)は紫外線を含む人工太陽光と紫外線をカットした人工太陽光の間で違いは見られなかった。また、血液像、組織学的観察においても両者の間に顕著な違いは見られなかった。

Effects of ultraviolet light (UV) irradiation on the skin physiology have been studied well. However, other effects than the skin physiology have not been investigated so much. Therefore, in this study, we examined the effects of UV irradiation on the immune and endocrine organs in hairless mice. Female hairless mice(Hr-/Kud; 8 weeks old)were used as a material. An artificial solar lamp (VITA-LITE; Light Sources Co. Ltd.) that simulates solar spectra including UV was used as a light source and a sheet of film (Acryplen: Mitsubishi Rayon) was used to remove UV. The animals were kept in a light and temperature controlled room (12 hour light and 12 hour darkness at 25 ℃). Significantly greater cyclobutane pyrimidine dimmers (CPDs) were detected in the skin of hairless mice when exposed to the solar simulated light with radiation (VITA-LITE) than that of animals exposed to the solar simulated light without UV. Organ weights (thymus, spleen, ovary, adrenal gland and liver) did not show statistical differences between the animals exposed to the solar simulated light with and without UV. Histological observation of these organs has not shown differences between the animals exposed to the solar simulated radiation with and without UV. Thus, the intensity of UV radiation used in the present study was strong enough to induce DNA damage in the skin, but it did not induce histological changes in immune and endocrine organs.

Journal

  • Bulletin of studies

    Bulletin of studies (14), 15-21, 2006

    Nara Saho College

Codes

  • NII Article ID (NAID)
    110006349554
  • NII NACSIS-CAT ID (NCID)
    AA11605938
  • Text Lang
    ENG
  • Journal Type
    大学紀要
  • ISSN
    13485911
  • NDL Article ID
    8808413
  • NDL Source Classification
    ZV1(一般学術誌--一般学術誌・大学紀要)
  • NDL Call No.
    Z22-1392
  • Data Source
    NDL  NII-ELS 
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