Promotion of Large Protein Crystal Growth with Stirring Solution

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Author(s)

Abstract

We propose a protein crystal growth with stirring the solution, which is entirely different approach compared with the conventional method. The novel method, which we call the Floating And Stirring Technique (FAST), enables both growing crystals on an insoluble and very dense liquid without contacting the vessel and stirring the protein solution mildly. Using this method together with slow cooling, we obtained a 3.0 mm-long lysozyme crystal in 20 days from a seed crystal. This is larger than the 2.0 mm-long crystal grown without stirring. Stirring the protein solution accelerates the growth of the protein crystal and prevents additional spontaneous nucleation.

Journal

  • Japanese Journal of Applied Physics

    Japanese Journal of Applied Physics 41(10), L1025-L1027, 2002-10-01

    The Japan Society of Applied Physics

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Codes

  • NII Article ID (NAID)
    110006350812
  • NII NACSIS-CAT ID (NCID)
    AA10650595
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • Journal Type
    大学紀要
  • ISSN
    00214922
  • NDL Article ID
    6306839
  • NDL Source Classification
    ZM35(科学技術--物理学)
  • NDL Call No.
    Z54-J337
  • Data Source
    CJP  CJPref  NDL  NII-ELS  J-STAGE 
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