Effects of Phenyl Derivatives of Dihydropyrazines with Ability to Generate Radical Species on Escherichia coli

• Takeda Osamu

  Department of Public Health, Faculty of Medicine, University of Miyazaki

• Takechi Shinji

  Faculty of Pharmaceutical Sciences, Sojo University

• Ito Shigeru

  Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University

• Omori Hisamitsu

  Kumamoto Health Center, Japanese Red Cross

• Katoh Takahiko

  Department of Public Health, Faculty of Medicine, University of Miyazaki

• Yamaguchi Tadatoshi

  Faculty of Pharmaceutical Sciences, Sojo University

抄録

Phenyl-substituted dihydropyrazines (Ph-DHPs) are derivatives of 2,3-dihydro-5,6-dimethylpyrazine (Me-DHP). Upon the addition of Cu²⁺, Me-DHP inhibits the growth of Escherichia coli by generating hydroxyl and carbon-centered radicals that cause DNA strand breakage. Here, we investigated the toxic effect of Ph-DHPs in several DNA repair-deficient or detoxifying enzyme-deficient mutant strains. Ph-DHPs caused cytotoxic and genotoxic damage, but, in a sodA sodB strain, the effects in the presence or absence of Cu²⁺ were different than those of Me-DHP. Our results suggest that the action of the generated superoxide anion in the interior side of the cell is remarkable.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 30 (9), 1663-1667, 2007

  公益社団法人 日本薬学会

キーワード

• dihydropyrazine
• superoxide anion
• lethal effect
• <i>Escherichia coli</i>

詳細情報

• CRID: 1390001204628421120
• NII論文ID: 110006380246
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.30.1663
• COI: 1:CAS:528:DC%2BD2sXhtFels7%2FK
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 8877755
• PubMed: 17827717
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8877755
• Web Site: http://www.jstage.jst.go.jp/article/bpb/30/9/30_9_1663/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2008127351
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可