Synergistic Effect of Kinetin and Benzyl Adenine Plays a Vital Role in High Frequency Regeneration from Cotyledon Explants of Bottle Gourd (Lagenaria siceraria) in Relation to Ethylene Production

  • Saha Shyamali
    Laboratory of Plant Genetics and Breeding, Graduate School of Bioagricultural Sciences, Nagoya University
  • Mori Hitoshi
    Laboratory of Developmental Signaling Biology, Graduate School of Bioagricultural Sciences, Nagoya University
  • Hattori Kazumi
    Laboratory of Plant Genetics and Breeding, Graduate School of Bioagricultural Sciences, Nagoya University

Search this article

Abstract

The interaction between plant hormones throughout plant regeneration and development is complex. In the present study, we extended our investigation and disclosed the capacity of de novo shoot organogenesis from cotyledon explants of bottle gourd in relation to plant hormones and ethylene inhibitor. Synergistic effect of kinetin and benzyl adenine (BA) enhanced the shoot regeneration efficiency (80.6%) from cotyledon explants of bottle gourd, compared with BA or kinetin used separately, even in the presence of AgNO3. The use of BA or kinetin separately or in synergistic combination led to a characteristic brownish color of the explants at the bud formation stage which was related to ethylene production. In the cotyledon explants cultured on MS medium supplemented with kinetin, the production of ethylene was lower than when BA or the combination of BA and kinetin was used. However, the regeneration capacity was very low and the emergence of shoot buds was delayed. BA promoted ethylene production while the synergistic effect of kinetin and BA decreased ethylene production and played a role in high frequency of regeneration from cotyledon explants of bottle gourd. The physiological relevance of the hormonal interaction in relation to the regulation of ethylene action and effect on regeneration was discussed.<br>

Journal

  • Breeding Science

    Breeding Science 57 (3), 197-202, 2007

    Japanese Society of Breeding

References(41)*help

See more

Details 詳細情報について

Report a problem

Back to top