魚類アレルゲンの分離とその性質

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  • ギョルイ アレルゲン ノ ブンリ ト ソノ セイシツ

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Abstract

Salt-soluble fractions (I, II) , an alkali-soluble fraction (III) and an acid-soluble fraction (IV) were separated from fresh Cod (Gadus macrocephalus). Allergenic activities of the I～IV fractions were measured using mAb parvalbumin (mAb PV) and sera of fish-allergy patients. 36kDa and 12.3kDa proteins of the fractions showed a positive reaction against mAb PV. It was presumed that the 12.3kDa protein was parvalbumin (PV) judging from references. However, a positive reaction was indicated only in the 36kDa protein of the III fraction in the IgE reaction with a fish-allergy patient serum. Therefore, it was presumed that the patient having the above serum developed an allergy to a protein being different from PV. Moreover, it was presumed that the 36kDa allergen has the immunity intersection to PV because the positive reaction was shown against mAb PV. Allergenic activities of alkali-soluble fractions (III) from Salmon, (Oncorhynchus keta), Saury, (Cololabis saira), Sardine (Sardinops melanostictus), Flatfish (Pleuronectes herzensteini) were measured with mAb PV and the sera of the patients. However, a clear allergen was not observed as compared with the Cod III fraction. The Cod III fraction in the acidic solution (pH2～6) was decreased about 50% binding activity with mAb PV by heating at 100℃. The activity of the same fraction in the alkaline solution (pH7～10) was not decreased. Therefore, the Cod III fraction was considered to be heat-stable in the alkaline solution.

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