ヒアルロン酸およびコラーゲン注入後の皮膚組織反応の検討  [in Japanese] Comparative study of skin tissue reactions after hyaluronic acid or collagen injection  [in Japanese]

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Author(s)

    • 安田廣生 Yasuda Hiroo
    • 金沢医科大学機能再建外科学 Department of Plastic and Reconstruction Surgery, Kanazawa Medical University

Abstract

目的:近年,浅い陥凹性瘢痕や顔面の皺などに対し注入療法がとられている。注入療法における臨床的な知見はこれまで多く報告されているが,注入による組織反応やその分解・吸収過程についての報告は少ない。本研究では注入剤(以下Fillerと称す)として使用されているコラーゲン製剤とヒアルロン酸製剤注入後の組織反応を比較検討した。対象と方法:非動物性安定化ヒアルロン酸のRestylane^[○!R]およびRestylane Perlane^[○!R]と牛真皮由来架橋コラーゲンのZyplast^[○!R]の3種類各々を,日本産白色家兎の耳介耳孔面皮下に注入し,その組織反応を経時的に観察した。結果:ヒアルロン酸群において注入早期に好酸球を主とする急性炎症細胞浸潤が観察されたが,この炎症は注入14日目以降には消失し,ヒアルロン酸は安定した状態を示した。60日目以降より異物巨細胞によるヒアルロン酸の貪食吸収像がみられ,注入180日目後では大部分が吸収された。一方コラーゲン群においては,早期より細胞浸潤が認められ,その後も継続して線維芽細胞の遊走・増殖,血管新生がみられた。同150日目には強い炎症細胞浸潤を伴う肉芽形成が認められ,ヒアルロン酸製剤に比し終始強い組織反応を示した。結論:理想的なFillerは安全性,効果的,生体適合性,非免疫原性,長期安定性,低コスト,吸収性を満たすとするならば,本研究によってヒアルロン酸製剤は,コラーゲン製剤に比し組織反応が軽微な点でより理想的製剤に近いと考えられた。

Purpose : Infusion therapy has recently been applied for slight dip-related scarring or facial wrinkles. Clinical knowledge about infusion therapy has increased, but few reports have described tissue reactions following injection and the disintegration/absorption process. We compared tissue reactions after injection between hyaluronic acid and a collagen drug used as infusion 'Filler' in this study. Subject and Methods : Using 3 types of filler (Restylane and Restylane Perlane as non-animal stabilization hyaluronic acid and Zyplast as cross-linked bovine dermal collagen), Japanese white rabbits were subcutaneously injected in the auricular concha and histological and immunological investigations were performed. Results : Acute inflammation with infiltration of numerous eosinophils was seen following hyaluronic acid injection in the early stages, but inflammation disappeared by day 14 after injection and hyaluronic acid was present in the tissue thereafter. An englobement absorption image by foreign body giant cells of hyaluronic acid was more marked 60 days after application and most were absorbed by 180 days. Conversely, in the group with collagen injection, cell infiltration was seen during the early stage, and continued thereafter, with histological examination revealing migration/increased fibroblasts and angiogenesis. Granulation tissue formation with marked infiltratation of inflammatory cells was seen up to 150 days, suggesting strong tissue reaction during observation. Conclusions : Ideal filler satisfies the conditions of safety, efficacy, biocompatibility, non-immunogenicity, long-term stability, low cost and absorbency. As for hyaluronic acid filler, tissue reaction was relatively weak, making this approach comparatively ideal compared to collagen-type filler.

Journal

  • Journal of Kanazawa Medical University

    Journal of Kanazawa Medical University 31, 233-241, 2006

    Kanazawa Medical University

Cited by:  3

Codes

  • NII Article ID (NAID)
    110006427062
  • NII NACSIS-CAT ID (NCID)
    AN00043827
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    03855759
  • Data Source
    CJPref  NII-ELS 
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