LIF- and IL-6-Induced Acetylation of STAT3 at Lys-685 through PI3K/Akt Activation

• Ohbayashi Norihiko

  Department of Immunology, Graduate School of Pharmaceutical Sciences Hokkaido University

• Ikeda Osamu

  Department of Immunology, Graduate School of Pharmaceutical Sciences Hokkaido University

• Taira Naohisa

  Department of Immunology, Graduate School of Pharmaceutical Sciences Hokkaido University

• Yamamoto Yu

  Department of Immunology, Graduate School of Pharmaceutical Sciences Hokkaido University

• Muromoto Ryuta

  Department of Immunology, Graduate School of Pharmaceutical Sciences Hokkaido University

• Sekine Yuichi

  Department of Immunology, Graduate School of Pharmaceutical Sciences Hokkaido University

• Sugiyama Kenji

  Nippon Boehringer Ingelheim Co., Ltd., Kawanishi Pharma Research Institute

• Honjoh Tsutomu

  Morinaga Institute of Biological Sciences, Inc.

• Matsuda Tadashi

  Department of Immunology, Graduate School of Pharmaceutical Sciences Hokkaido University

抄録

Signal transducer and activator of transcription 3 (STAT3), which mediates biological actions in many physiological processes, is activated by cytokines and growth factors via specific tyrosine or serine phosphorylation, dimerization and nuclear translocation. A recent study has demonstrated, by using antibody to acetylated lysine, and a STAT3 mutant with Lys-685-to-Arg substitution, that STAT3 is acetylated at Lys-685 by histone acetyltransferase p300, and that acetylation at Lys-685 is critical for STAT3 activation. In the present study, we created an acetyl-specific antibody against STAT3 acetylated at Lys-685, and found that leukemia inhibitory factor (LIF) or interleukin (IL)-6 induced acetylation of STAT3 at Lys-685 in 293T and Hep3B cells. Moreover, acetylation of STAT3 at Lys-685 was suppressed by PI3K inhibitor LY294002, or a dominant negative Akt. Taken together, our findings demonstrate that endogenous STAT3 is acetylated at Lys-685 by LIF or IL-6 through PI3K/Akt activation.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 30 (10), 1860-1864, 2007

  公益社団法人 日本薬学会

キーワード

• signal transducer and activator of transcription 3
• interleukin-6
• acetylation
• leukemia inhibitory factor
• PI3K
• Akt

詳細情報

• CRID: 1390001204625060736
• NII論文ID: 110006436364
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.30.1860
• ISSN: 13475215
• ISSN: 09186158
• HANDLE: 2115/28780
• NDL書誌ID: 8919008
• Web Site: http://id.ndl.go.jp/bib/8919008
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8919008
• Web Site: http://www.jstage.jst.go.jp/article/bpb/30/10/30_10_1860/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2008096837
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可