Free Radical Scavenger Edaravone Suppresses X-ray-induced Apoptosis through p53 Inhibition in MOLT-4 Cells

Author(s)

    • KATSUMURA Yosuke
    • Nuclear Engineering Research Laboratory, Graduate School of Engineering, The University of Tokyo
    • MATSUMOTO Yoshihisa
    • Section of Radiation Biology, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, The University of Tokyo
    • MIYAGAWA Kiyoshi
    • Section of Radiation Biology, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, The University of Tokyo
    • IGAKI Hiroshi
    • Department of Radiology, Graduate School of Medicine, The University of Tokyo
    • NAKAGAWA Keiichi
    • Department of Radiology, Graduate School of Medicine, The University of Tokyo

Abstract

Edaravone, a clinical drug used widely for the treatment of acute cerebral infarction, is reported to scavenge free radicals. In the present study, we investigated the radioprotective effect of edaravone on X-ray-induced apoptosis in MOLT-4 cells. Apoptosis was determined by the dye exclusion test, Annexin V binding assay, cleavage of caspase, and DNA fragmentation. We found that edaravone significantly suppressed the X-ray-induced apoptosis. The amount of intracellular ROS production was determined by the chloromethyl-2',7'-dichlorodihydro-fluorescein diacetate system. We found that the intracellular ROS production by X-irradiation was completely suppressed by the addition of edaravone. The accumulation and phosphorylation of p53 and the expression of p21<sup>WAF1</sup>, a target protein of p53, which were induced by X-irradiation, were also suppressed by adding edaravone. We conclude that the free radical scavenger edaravone suppresses X-ray-induced apoptosis in MOLT-4 cells by inhibiting p53.<br>

Journal

  • Journal of Radiation Research

    Journal of Radiation Research 48(6), 495-503, 2007-12-15

    Journal of Radiation Research Editorial Committee

References:  58

Cited by:  1

Codes

  • NII Article ID (NAID)
    110006452332
  • NII NACSIS-CAT ID (NCID)
    AA00705792
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    04493060
  • NDL Article ID
    9256544
  • NDL Source Classification
    ZS45(科学技術--医学--放射線医学)
  • NDL Call No.
    Z53-P232
  • Data Source
    CJP  CJPref  NDL  NII-ELS  J-STAGE  NDL-Digital 
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