Free Radical Scavenger Edaravone Suppresses X-ray-induced Apoptosis through p53 Inhibition in MOLT-4 Cells
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- Sasano Nakashi SASANO Nakashi
- Department of Radiology, Graduate School of Medicine, The University of Tokyo
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- Enomoto Atsushi ENOMOTO Atsushi
- Section of Radiation Biology, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, The University of Tokyo
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- Hosoi Yoshio [他] HOSOI Yoshio
- Section of Radiation Biology, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, The University of Tokyo
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- KATSUMURA Yosuke
- Nuclear Engineering Research Laboratory, Graduate School of Engineering, The University of Tokyo
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- MATSUMOTO Yoshihisa
- Section of Radiation Biology, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, The University of Tokyo
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- SHIRAISHI Kenshiro
- Department of Radiology, Graduate School of Medicine, The University of Tokyo
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- MIYAGAWA Kiyoshi
- Section of Radiation Biology, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, The University of Tokyo
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- IGAKI Hiroshi
- Department of Radiology, Graduate School of Medicine, The University of Tokyo
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- NAKAGAWA Keiichi
- Department of Radiology, Graduate School of Medicine, The University of Tokyo
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Author(s)
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- Sasano Nakashi SASANO Nakashi
- Department of Radiology, Graduate School of Medicine, The University of Tokyo
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- Enomoto Atsushi ENOMOTO Atsushi
- Section of Radiation Biology, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, The University of Tokyo
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- Hosoi Yoshio [他] HOSOI Yoshio
- Section of Radiation Biology, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, The University of Tokyo
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- KATSUMURA Yosuke
- Nuclear Engineering Research Laboratory, Graduate School of Engineering, The University of Tokyo
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- MATSUMOTO Yoshihisa
- Section of Radiation Biology, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, The University of Tokyo
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- SHIRAISHI Kenshiro
- Department of Radiology, Graduate School of Medicine, The University of Tokyo
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- MIYAGAWA Kiyoshi
- Section of Radiation Biology, Center for Disease Biology and Integrative Medicine, Faculty of Medicine, The University of Tokyo
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- IGAKI Hiroshi
- Department of Radiology, Graduate School of Medicine, The University of Tokyo
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- NAKAGAWA Keiichi
- Department of Radiology, Graduate School of Medicine, The University of Tokyo
Abstract
Edaravone, a clinical drug used widely for the treatment of acute cerebral infarction, is reported to scavenge free radicals. In the present study, we investigated the radioprotective effect of edaravone on X-ray-induced apoptosis in MOLT-4 cells. Apoptosis was determined by the dye exclusion test, Annexin V binding assay, cleavage of caspase, and DNA fragmentation. We found that edaravone significantly suppressed the X-ray-induced apoptosis. The amount of intracellular ROS production was determined by the chloromethyl-2',7'-dichlorodihydro-fluorescein diacetate system. We found that the intracellular ROS production by X-irradiation was completely suppressed by the addition of edaravone. The accumulation and phosphorylation of p53 and the expression of p21<sup>WAF1</sup>, a target protein of p53, which were induced by X-irradiation, were also suppressed by adding edaravone. We conclude that the free radical scavenger edaravone suppresses X-ray-induced apoptosis in MOLT-4 cells by inhibiting p53.<br>
Journal
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- Journal of Radiation Research
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Journal of Radiation Research 48(6), 495-503, 2007-12-15
Journal of Radiation Research Editorial Committee
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