Simultaneous Accumulation of High Contents of α-Tocopherol and Lutein is Possible in Seeds of Soybean (Glycine max (L.) Merr.)

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Author(s)

    • ABE Jun
    • Laboratory of Plant Genetics and Evolution, Graduate School of Agriculture, Hokkaido University
    • YAMADA Tetsuya
    • Laboratory of Plant Genetics and Evolution, Graduate School of Agriculture, Hokkaido University
    • KITAMURA Keisuke
    • Laboratory of Plant Genetics and Evolution, Graduate School of Agriculture, Hokkaido University

Abstract

Soybean is rich in various functional components such as isoflavones, saponins, tocopherols and sterols that are beneficial to human health. However, the contents of some of these components such as α-tocopherol (α-Toc) and lutein are relatively low in the seeds. Therefore, genetic manipulation to enhance the α-Toc and lutein contents is an important breeding objective in soybean. Recently, we have successfully identified soybean varieties and wild strains with high α-Toc and lutein contents, respectively. In the present investigation, we made a cross between a soybean variety with a high α-Toc content thereafter referred to as a high α-Toc soybean variety, Keszthelyi A.S. (female parent), and a wild soybean strain with a high lutein content thereafter referred to as a high lutein wild soybean strain, B09092 (pollen parent). We analyzed the α-Toc and lutein contents of the F_2 seed and F_2-plant (F_3 seeds) populations by a single-pass method for extraction and quantification, using high performance liquid chromatography (HPLC). The α-Toe content of the F_2 seeds ranged from 1.0 to 6.0mg/100g meal, whereas that of the high α-Toc parent, var. Keszthelyi A.S. varied from 4.27 to 6.43mg/100g meal, and that of the wild soybean parent, B09092 ranged from 1.74 to 2.30mg/100g meal. The lutein content of the F_2 seeds ranged from 0.1 to 4.0mg/100g meal, while that of the var. Keszthelyi A.S. from 0.19 to 0.42mg/100g meal, and that of the high lutein parent, B09092 from 2.48 to 3.50mg/100g meal. Broad-sense heritability for the α-Toc and lutein contents in the F_2 seeds was estimated to be 0.598 and 0.656, respectively. In the F_2-plants (F_3 seeds), the broad-sense heritabilities were 0.693 for the α-Toc content and 0.824 for the lutein content, respectively. The heritabilities in a narrow sense for the α-Toc and lutein contents in the F_2 seeds were 0.598 and 0.413, respectively. In the F_2-plants (F_3 seeds), the estimates were 0.693 and 0.718, respectively. The results suggested that both high α-Toc and lutein contents are highly heritable. A significant positive correlation was observed between the α-Toc and lutein contents both in the F_2 seeds (r=0.420, P<0.01) and in the F_2-plants (F_3 seeds)(r=0.228, P<0.01). In addition, a significant positive genetic correlation was observed between the α-Toc and lutein traits in the F_2-plants (F_3 seeds)(r_G=0.311, P<0.01). No correlation was observed between the 100-kernel weight and lutein content in the F_2-plants (F_3 seeds). Likewise no correlation was obtained between the 100-kernel weight and α-Toc content. These results indicate that it is possible to simultaneously select individuals among the progenies with high contents of lutein and α-Toc without decreasing the kernel weight of the seeds.

Journal

  • Breeding science

    Breeding science 57(4), 297-304, 2007-12-01

    Japanese Society of Breeding

References:  30

Cited by:  1

Codes

  • NII Article ID (NAID)
    110006473838
  • NII NACSIS-CAT ID (NCID)
    AA11353132
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13447610
  • NDL Article ID
    9282635
  • NDL Source Classification
    ZR6(科学技術--農林水産)
  • NDL Call No.
    Z54-J372
  • Data Source
    CJP  CJPref  NDL  NII-ELS 
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