時間分解蛍光測定法を用いたブタ血中LH濃度の測定

• NOGUCHI Michiko

  Laboratory of Veterinary Internal Medicine, Azabu University

• YOSHIOKA Koji

  National Institute of Animal Health

• KANEKO Hiroyuki

  National Institute of Agrobiological Sciences

• IWAMURA Shokichi

  National Institute of Animal Health

• TAKAHASHI Toru

  National Institute of Agrobiological Sciences

• SUZUKI Chie

  National Institute of Animal Health

• ARAI Sachiko

  Laboratory of Veterinary Internal Medicine, Azabu University

• WADA Yasunori

  Laboratory of Veterinary Internal Medicine, Azabu University

• ITOH Seigo

  Laboratory of Veterinary Internal Medicine, Azabu University

書誌事項

タイトル別名

• Measurement of Porcine Luteinizing Hormone Concentration in Blood by Time-Resolved Fluoroimmunoassay
• Theriogenology: Measurement of porcine luteinizing hormone concentration in blood by time-resolved fluoroimmunoassay

抄録

We validated a time-resolved fluoroimmunoassay (TR-FIA) using the lanthanide element europium as a non-radiological tracer for measuring luteinizing hormone (LH) in porcine peripheral blood. The dose-response curve of the reference standard ranged from 0.2048 to 50 ng/ml. Good parallelism was noted between the LH standard and plasma sample. The profile of LH throughout the estrous cycle, assessed by daily blood sampling, was consistent with the previous findings obtained by radioimmunoassays (RIAs). Moreover, the secretory patterns of pulsatile LH in the follicular phase and the preovulatory LH surge were also similar to those obtained in previous RIAs. We conclude that TR-FIA can be used to measure LH levels in porcine blood, with practical and convenient applications.<br>

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 69 (12), 1291-1294, 2007

  公益社団法人 日本獣医学会

キーワード

• LH
• Pig
• TR-FIA

詳細情報

• CRID: 1390282681404738816
• NII論文ID: 110006569955
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.69.1291
• COI: 1:CAS:528:DC%2BD1cXhtFKrsro%3D
• ISSN: 13477439
• ISSN: 09167250
• NDL書誌ID: 9319091
• PubMed: 18176028
• Web Site: https://agriknowledge.affrc.go.jp/RN/2010760095
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9319091
• Web Site: http://www.jstage.jst.go.jp/article/jvms/69/12/69_12_1291/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2008105921
• 本文言語コード: en
• データソース種別:
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  • IRDB
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可